USE1 Human

Unconventional SNARE In The ER 1 Human Recombinant
Cat. No.
BT28613
Source
Escherichia Coli.
Synonyms
Vesicle transport protein USE1, Putative MAPK-activating protein PM26, USE1-like protein, p31, USE1L, MDS032, Q-SNARE, SLT1, SNARE-Like Tail-Anchored Protein 1 Homolog, Protein P31.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

USE1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-231 a.a) and having a molecular mass of 28.3kDa.
USE1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
USE1 is a vesicle transport protein belonging to the USE1 family. It forms a SNARE complex with STX18, USE1L, BNIP1/SEC20L, and SEC22B and interacts directly with STX18. This SNARE complex is potentially involved in the targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. USE1 has been linked to diseases like dysentery and hemolytic-uremic syndrome. Its associated super-pathways include the Nicotine Pathway (Dopaminergic Neuron) and Pharmacodynamics. GO annotations for this gene encompass protein binding.
Description
Recombinant human USE1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 254 amino acids, including a 23 amino acid His-tag at the N-terminus, and has a molecular weight of 28.3 kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The USE1 protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the USE1 protein is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Vesicle transport protein USE1, Putative MAPK-activating protein PM26, USE1-like protein, p31, USE1L, MDS032, Q-SNARE, SLT1, SNARE-Like Tail-Anchored Protein 1 Homolog, Protein P31.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAASRLE LNLVRLLSRC EAMAAEKRDP DEWRLEKYVG ALEDMLQALK VHASKPASEV INEYSWKVDF LKGMLQAEKL TSSSEKALAN QFLAPGRVPT TARERVPATK TVHLQSRARY TSEMRSELLG TDSAEPEMDV RKRTGVAGSQ PVSEKQSAAE LDLVLQRHQN LQEKLAEEML GLARSLKTNT LAAQSVIKKD NQTLSHSLKM ADQNLEKLKT ESERLEQHTQ KSVN

Product Science Overview

Function and Mechanism

USE1 is involved in the retrograde transport of vesicles from the Golgi apparatus back to the ER . This process is essential for maintaining the proper function and organization of the ER and Golgi network. The protein acts as a SNARE that facilitates the targeting and fusion of Golgi-derived vesicles with the ER . This function is critical for the recycling of vesicular components and the maintenance of cellular homeostasis.

Structure

The USE1 protein is characterized by its SNARE motif, which is a conserved sequence that allows it to interact with other SNARE proteins to form a complex. This complex is necessary for the fusion of vesicles with target membranes . The protein also contains a tail-anchor that helps it embed into the membrane of the ER .

Clinical Significance

Mutations or dysregulation of the USE1 gene have been associated with various diseases, including pericardium leiomyoma and chest wall lymphoma . Additionally, the protein’s role in vesicle transport and lysosomal function suggests that it may be involved in other cellular processes and pathologies .

Research and Applications

Recombinant USE1 protein is used in various research applications to study its function and role in cellular processes. It is typically produced in a laboratory setting using recombinant DNA technology, which involves inserting the USE1 gene into an expression system to produce the protein in large quantities . This recombinant protein can then be used in biochemical assays, structural studies, and other experimental setups to gain insights into its function and mechanism.

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