UNC119B Human
UNC-119 Homolog B Human Recombinant
Cat. No.
BT28387
Source
E.coli.
Synonyms
Unc-119 homolog B (C. elegans), POC7B, POC7 centriolar protein homolog B, MGC5139.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
UNC119B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 271 amino acids (1-251) and having a molecular mass of 30.3kDa.
UNC119B is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
UNC119B is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
UCN119B, a member of the PDE6D/unc-119 family, is found in high concentrations in the photoreceptors of the retina. This protein shares significant similarities with the C. elegans unc119 gene and can compensate for its absence in mutated C. elegans organisms.
Description
Recombinant human UNC119B, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 271 amino acids (1-251) with a molecular weight of 30.3 kDa. This protein is expressed with an N-terminal 20 amino acid His-tag and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The UNC119B solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 100mM NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Unc-119 homolog B (C. elegans), POC7B, POC7 centriolar protein homolog B, MGC5139.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSGSNPKAAA AASAAGPGGL VAGKEEKKKA GGGVLNRLKA RRQAPHHAAD DGVGAAVTEQ ELLALDTIRP EHVLRLSRVT ENYLCKPEDN IYSIDFTRFK IRDLETGTVL FEIAKPCVSD QEEDEEEGGG DVDISAGRFV RYQFTPAFLR LRTVGATVEF TVGDKPVSNF RMIERHYFRE HLLKNFDFDF GFCIPSSRNT CEHIYEFPQL SEDVIRLMIE NPYETRSDSF YFVDNKLIMH NKADYAYNGG Q
Product Science Overview
Gene and Protein Structure
The UNC119B gene encodes a protein that is composed of 251 amino acids. The recombinant human UNC119B protein is often expressed in Escherichia coli and purified using conventional chromatography techniques . The protein is typically tagged with a His-tag at the N-terminus to facilitate purification and detection .
Functional Role
UNC119B plays a crucial role in the retina, where it is involved in the function of photoreceptor cells. It can functionally complement the C. elegans unc119 mutation gene, indicating its evolutionary conservation and importance . The protein is associated with the mammalian multisubunit photoreceptor cGMP phosphodiesterase PDE alpha beta gamma 2 (PDE6 family), which is a peripherally membrane-associated enzyme .
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