ULBP1 Human, Sf9

UL16 Binding Protein 1 Human Recombinant, Sf9
Cat. No.
BT23811
Source
Sf9, Baculovirus cells.
Synonyms
UL16 Binding Protein 1, Retinoic Acid Early Transcript 1I, alcan-beta, NKG2D Ligand 1, NKG2DL1, RAET1I, N2DL-1, NKG2D ligand 1, ULBP.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ULBP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 200 amino acids (26-216) and having a molecular mass of 23.4kDa (Molecular size on SDS-PAGE will appear at approximately 20-40kDa).
ULBP1 is fused to a 6 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
ULBP1, along with ULBP2 and ULBP3, serves as a ligand for the NKG2D receptor. These ULBPs activate various signaling pathways in primary NK cells, leading to the production of cytokines and chemokines. In cells infected with CMV, ULBP1 interacts with the soluble CMV glycoprotein UL16 to inhibit binding to the NKG2D receptor, enabling CMV-infected cells to evade the immune system. Furthermore, UL16 retains ULBP1 within the ER and cis-Golgi apparatus, preventing its transport to the cell surface.
Description
Recombinant human ULBP1, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. It consists of 200 amino acids (residues 26-216) and has a molecular mass of 23.4 kDa. On SDS-PAGE, the apparent molecular size will be approximately 20-40 kDa due to glycosylation. ULBP1 is fused to a 6-amino acid IgG His-tag at its C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
ULBP1 protein is supplied as a 0.5 mg/ml solution in phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store at 4°C. For long-term storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
UL16 Binding Protein 1, Retinoic Acid Early Transcript 1I, alcan-beta, NKG2D Ligand 1, NKG2DL1, RAET1I, N2DL-1, NKG2D ligand 1, ULBP.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADLGWVDTHC LCYDFIITPK SRPEPQWCEV QGLVDERPFL HYDCVNHKAK AFASLGKKVN VTKTWEEQTE TLRDVVDFLK GQLLDIQVEN LIPIEPLTLQ ARMSCEHEAH GHGRGSWQFL FNGQKFLLFD SNNRKWTALH PGAKKMTEKW EKNRDVTMFF QKISLGDCKM WLEEFLMYWE QMLDPTKPPS LAPGHHHHHH.

Product Science Overview

Structure and Function

The structure of ULBP1 consists of the alpha-1 and alpha-2 domains, which are connected to the cell membrane via the GPI anchor . This protein plays a crucial role in the immune system by binding and activating the KLRK1/NKG2D receptor on natural killer (NK) cells and some T-cells . This interaction mediates NK cell cytotoxicity, which is essential for the immune response against infected or transformed cells .

Interaction with Viral Glycoproteins

ULBP1 also interacts with viral glycoproteins, such as the UL16 glycoprotein from cytomegalovirus (CMV) . This interaction helps the virus evade the immune system by retaining ULBP1 in the endoplasmic reticulum, preventing it from activating NK cells .

Recombinant Production in Sf9 Cells

The recombinant form of ULBP1 is produced in Sf9 Baculovirus cells . This method allows for the production of a single, glycosylated polypeptide chain that retains the functional properties of the native protein . The recombinant ULBP1 produced in Sf9 cells is used in various research applications, including studies on immune response and viral evasion mechanisms .

Applications in Research

ULBP1 is widely used in research to study its role in the immune system and its interactions with viral proteins. It is also used to investigate the mechanisms of NK cell activation and the pathways involved in immune responses . Additionally, recombinant ULBP1 is valuable for developing therapeutic strategies targeting immune evasion by viruses and enhancing NK cell-mediated cytotoxicity .

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UL16 Binding Protein 1 Human Recombinant
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