UGDH Antibody

UDP-Glucose Dehydrogenase (UGDH) is a crucial enzyme involved in the biosynthesis of glycosaminoglycans, which are essential components of the extracellular matrix. This enzyme catalyzes the oxidation of UDP-glucose to UDP-glucuronic acid, a key precursor in the synthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate . These glycosylated compounds play significant roles in cellular processes including signal transduction, cell migration, and cancer growth and metastasis .

UGDH is a NAD±dependent enzyme that performs a double oxidation of UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA) while reducing NAD+ to NADH . The enzyme is encoded by the UGDH gene, which is located on chromosome 4 in humans and chromosome 5 in mice . The protein structure of UGDH includes several domains responsible for its catalytic activity and binding to NAD+ .

The product of the UGDH-catalyzed reaction, UDP-glucuronic acid, is utilized in various metabolic pathways, including the biosynthesis of glycosaminoglycans and the detoxification of xenobiotics in hepatocytes . Glycosaminoglycans are vital for maintaining the structural integrity of tissues and facilitating cellular communication . Additionally, UGDH activity is regulated by various factors, including transforming growth factor-beta (TGF-β) and hypoxia .

Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins. These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various immunoassays, including ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry . Mouse anti-human antibodies can be conjugated with different labels such as HRP, FITC, and biotin to facilitate detection and signal amplification .

Mouse anti-human antibodies are widely used in research to detect, sort, and purify human proteins. They offer increased versatility and sensitivity in various detection systems . In the context of UGDH research, these antibodies can be used to study the expression and function of UGDH in human tissues and cells. By using mouse anti-human antibodies, researchers can gain insights into the role of UGDH in different biological processes and disease states.