UCHL5 Human

Ubiquitin Carboxyl-Terminal Esterase L5 Human Recombinant
Cat. No.
BT19995
Source
Escherichia Coli.
Synonyms
Ubiquitin Carboxyl-terminal Hydrolase L5, UCH37, CGI-70, UCH-L5, INO80R, Ubiquitin thioesterase L5, INO80 complex subunit R, EC 3.4.19.12, Ubiquitin Carboxyl-terminal Esterase L5.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UCHL5 produced in E.Coli is a single, non-glycosylated polypeptide chain containing349 amino acids (1-329a.a.) and having a molecular mass of 39.7kDa.
UCHL5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
UCHL5, a member of the peptidase C12 family, is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. This deubiquitinating enzyme is related to the 19S regulatory subunit of the 26S proteasome.
Description
Produced in E. coli, UCHL5 is a single, non-glycosylated polypeptide chain consisting of 349 amino acids (1-329a.a.) with a molecular mass of 39.7 kDa. It features a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The UCHL5 protein solution (0.5 mg/ml) is formulated in a 20 mM Tris-HCl buffer (pH 8.0) containing 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA, and 30% glycerol.
Purity
Purity exceeds 90% as determined by SDS-PAGE.
Biological Activity
Specific activity is greater than 500 pmole/min/µg, measured by the hydrolysis of Ubiquitin-AMC at pH 8.0 and 37°C.
Stability
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Synonyms
Ubiquitin Carboxyl-terminal Hydrolase L5, UCH37, CGI-70, UCH-L5, INO80R, Ubiquitin thioesterase L5, INO80 complex subunit R, EC 3.4.19.12, Ubiquitin Carboxyl-terminal Esterase L5.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK

Product Science Overview

Introduction

Ubiquitin Carboxyl-Terminal Esterase L5 (UCHL5), also known as Ubiquitin C-Terminal Hydrolase L5, is a protease that plays a crucial role in the ubiquitin-proteasome system. This enzyme is involved in the regulation of protein degradation, which is essential for maintaining cellular homeostasis. UCHL5 is encoded by the UCHL5 gene in humans and is associated with various biological processes and diseases.

Structure and Function

UCHL5 belongs to the peptidase C12 family and is known for its ability to specifically cleave ‘Lys-48’-linked polyubiquitin chains . This deubiquitinating enzyme is associated with the 19S regulatory subunit of the 26S proteasome, where it plays a role in the removal of ubiquitin from substrates targeted for degradation . The enzyme’s activity is crucial for the regulation of protein turnover and the prevention of the accumulation of damaged or misfolded proteins.

Biological Significance

UCHL5 is involved in several key biological processes, including:

  • Protein Deubiquitination: UCHL5 removes ubiquitin moieties from proteins, thereby regulating their stability and function .
  • Proteasomal Degradation: By interacting with the 19S regulatory subunit of the 26S proteasome, UCHL5 facilitates the degradation of ubiquitinated proteins .
  • Regulation of Signaling Pathways: UCHL5 has been implicated in the regulation of various signaling pathways, including the TGF-beta receptor signaling pathway and the Wnt/β-catenin pathway .
Clinical Relevance

UCHL5 has been associated with several diseases and pathological conditions:

  • Cancer: UCHL5 has been shown to promote the progression of hepatocellular carcinoma (HCC) by enhancing glycolysis through the activation of the Wnt/β-catenin pathway . High expression levels of UCHL5 are correlated with poor prognosis in HCC patients .
  • Diabetic Retinopathy: UCHL5 is also linked to background diabetic retinopathy and microvascular complications of diabetes .
Recombinant UCHL5

Recombinant UCHL5 is a form of the enzyme that is produced through recombinant DNA technology. This allows for the production of large quantities of the enzyme for research and therapeutic purposes. Recombinant UCHL5 is used in various biochemical assays to study its function and to screen for potential inhibitors that could be used as therapeutic agents.

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