UCHL3 Mouse
Description
UCHL3 is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Ubiquitin Carboxyl-Terminal Esterase L3 (UCHL3) is a member of the ubiquitin C-terminal hydrolase family, which plays a crucial role in the ubiquitin-proteasome system. This system is essential for protein degradation and regulation within the cell. UCHL3 is a deubiquitinating enzyme (DUB) that specifically hydrolyzes the peptide bond at the C-terminal glycine of ubiquitin, releasing monomeric ubiquitin from ubiquitin-protein conjugates .
The UCHL3 gene is located on chromosome 13q22.2 in humans and on a syntenic region on chromosome 14 in mice . The gene encodes a protein that is 230 amino acids in length with a predicted molecular weight of approximately 26 kDa . The protein contains a single N-terminal ubiquitin C-terminal hydrolase (UCH) domain, which is responsible for its enzymatic activity .
UCHL3 is widely expressed in various tissues, with the highest levels observed in the heart, testis, thymus, and striated muscle . It is involved in several cellular processes, including the liberation of monomeric ubiquitin from precursors encoded by ubiquitin genes and the recycling of ubiquitin monomers . This activity is crucial for maintaining the balance of ubiquitin within the cell, which is necessary for proper protein degradation and regulation .
The crystal structure of human UCHL3 has been determined, revealing insights into its enzymatic mechanism . The protein’s active site contains a short crossover loop, which allows it to process small ubiquitin derivatives efficiently . Recombinant UCHL3 protein has been shown to display UCH activity in vitro, confirming its role as a deubiquitinating enzyme .
Studies involving mouse models have provided valuable insights into the function of UCHL3. Mice homozygous for a targeted deletion of the Uchl3 gene are indistinguishable from wild-type mice, suggesting that UCHL3 may have redundant functions with other deubiquitinating enzymes . However, double homozygous mice for both Uchl1 and Uchl3 genes display more severe phenotypes, including earlier onset of lethality and axonal degeneration .
Recombinant UCHL3 proteins, such as those derived from E. coli, are widely used in research to study the enzyme’s function and its role in various cellular processes . These recombinant proteins are typically supplied in a solution containing HEPES, NaCl, and TCEP, and are stored at -70°C to maintain stability .
