UBXN2B Human
UBX Domain Protein 2B Human Recombinant
Cat. No.
BT28143
Source
Escherichia Coli.
Synonyms
UBX domain-containing protein 2B, p37, NSFL1 cofactor p37, p97 cofactor p37, UBXN2B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
UBXN2B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-331) and having a molecular mass of 39.5 kDa. UBXN2B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
UBXN2B (UBX Domain Protein 2B) is a protein composed of 331 amino acids. It contains both a UBX domain and a SEP domain. This protein plays a crucial role in the creation and upkeep of the endoplasmic reticulum (ER) and Golgi apparatus during the interphase stage of the cell cycle. It's also vital for their reconstruction after mitosis. UBXN2B interacts with VCP to form a complex that facilitates membrane fusion.
Description
Recombinant human UBXN2B, expressed in E.coli, is a single, non-glycosylated polypeptide chain consisting of 354 amino acids (residues 1-331). It has a molecular weight of 39.5 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The UBXN2B solution is provided at a concentration of 0.5mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 90.0% via SDS-PAGE analysis.
Synonyms
UBX domain-containing protein 2B, p37, NSFL1 cofactor p37, p97 cofactor p37, UBXN2B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEGGGP EPGEQERRSS GPRPPSARDL QLALAELYED EVKCKSSKSN RPKATVFKSP RTPPQRFYSS EHEYSGLNIV RPSTGKIVNE LFKEAREHGA VPLNEATRAS GDDKSKSFTG GGYRLGSSFC KRSEYIYGEN QLQDVQILLK LWSNGFSLDD GELRPYNEPT NAQFLESVKR GEIPLELQRL VHGGQVNLDM EDHQDQEYIK PRLRFKAFSG EGQKLGSLTP EIVSTPSSPE EEDKSILNAV VLIDDSVPTT KIQIRLADGS RLIQRFNSTH RILDVRNFIV QSRPEFAALD FILVTSFPNK ELTDESLTLL EADILNTVLL QQLK.
Product Science Overview
Structure and Domains
UBXN2B is a 331 amino acid protein that contains two significant domains:
- UBX Domain: This domain is involved in protein-protein interactions and is crucial for the protein’s function in cellular processes.
- SEP Domain: This domain is essential for the protein’s role in endoplasmic reticulum (ER) and Golgi biogenesis.
Biological Functions
UBXN2B is required for the biogenesis of the ER and Golgi apparatus. It plays a vital role in maintaining these organelles during interphase and their reassembly at the end of mitosis. The protein interacts with VCP (valosin-containing protein) to form a complex that has membrane fusion activity, which is essential for the proper functioning of the ER and Golgi .
Recombinant UBXN2B
Recombinant human UBXN2B protein is typically produced in Escherichia coli (E. coli) and purified using conventional chromatography techniques. The recombinant protein often includes an N-terminal His-tag to facilitate purification and detection. The recombinant form retains the functional domains and biological activity of the native protein, making it useful for research purposes .
Applications in Research
Recombinant UBXN2B is used in various research applications, including:
- Studying ER and Golgi Biogenesis: Researchers use recombinant UBXN2B to understand the mechanisms underlying the formation and maintenance of the ER and Golgi apparatus.
- Protein-Protein Interaction Studies: The UBX domain’s role in protein interactions makes recombinant UBXN2B valuable for studying these interactions in vitro.
- Membrane Fusion Studies: The interaction between UBXN2B and VCP and their role in membrane fusion can be studied using the recombinant protein .
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