Introduction
Ubiquilin-2, also known as UBQLN2, is a protein that plays a crucial role in the ubiquitin-proteasome system, which is responsible for degrading damaged or unnecessary proteins within cells. UBQLN2 contains two main domains: an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. These domains allow UBQLN2 to interact with both proteasomes, the cellular machinery responsible for protein degradation, and ubiquitin ligases, enzymes that tag proteins for destruction. By linking these components, UBQLN2 facilitates the degradation of targeted proteins. Additionally, UBQLN2 has been found to bind to the ATPase domain of Stch, a protein similar to heat shock protein 70 (Hsp70). Mutations in the UBQLN2 gene have been linked to neurological disorders, including amyotrophic lateral sclerosis 15 (ALS 15), which may or may not be accompanied by frontotemporal dementia, a condition affecting the frontal and temporal lobes of the brain.
Description
Recombinant UBQLN2 protein, produced in E. coli bacteria, is a purified protein consisting of a single polypeptide chain that is not glycosylated (does not contain sugar modifications). It encompasses 647 amino acids, representing amino acids 1 through 624 of the full-length human UBQLN2 protein. The recombinant protein has a molecular weight of 68.1 kDa and includes a 23-amino acid His-tag attached to its N-terminus. This His-tag facilitates purification using specialized chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The UBQLN2 protein solution has a concentration of 0.25 mg/ml and is formulated in a buffer containing phosphate-buffered saline (pH 7.4), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the UBQLN2 protein solution should be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as albumin (HSA or BSA) at a concentration of 0.1% is advisable. To maintain protein integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of the UBQLN2 protein is greater than 90%, as determined by SDS-PAGE analysis, a technique used to separate proteins based on their size.
Synonyms
UBQLN2,
Ubiquilin 2, CHAP1, PLIC2, Protein Linking IAP With Cytoskeleton 2,
Ubiquitin-Like Product Chap1/Dsk2, ALS15, N4BP4, NEDD4 Binding Protein 4,
Nedd4 Binding Protein 4, DSK2 Homolog, Ubiquilin-2, HRIHFB2157, HPLIC-2,
PLIC-2, DSK2.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAENGES SGPPRPSRGP
AAAQGSAAAP AEPKIIKVTV KTPKEKEEFA VPENSSVQQF KEAISKRFKS QTDQLVLIFA GKILKDQDTL
IQHGIHDGLT VHLVIKSQNR PQGQSTQPSN AAGTNTTSAS TPRSNSTPIS TNSNPFGLGS LGGLAGLSSL
GLSSTNFSEL QSQMQQQLMA SPEMMIQIME NPFVQSMLSN PDLMRQLIMA NPQMQQLIQR NPEISHLLNN
PDIMRQTLEI ARNPAMMQEM MRNQDLALSN LESIPGGYNA LRRMYTDIQE PMLNAAQEQF GGNPFASVGS
SSSSGEGTQP SRTENRDPLP NPWAPPPATQ SSATTSTTTS TGSGSGNSSS NATGNTVAAA NYVASIFSTP
GMQSLLQQIT ENPQLIQNML SAPYMRSMMQ SLSQNPDLAA QMMLNSPLFT ANPQLQEQMR PQLPAFLQQM
QNPDTLSAMS NPRAMQALMQ IQQGLQTLAT EAPGLIPSFT PGVGVGVLGT AIGPVGPVTP IGPIGPIVPF
TPIGPIGPIG PTGPAAPPGS TGSGGPTGPT VSSAAPSETT SPTSESGPNQ QFIQQMVQAL AGANAPQLPN
PEVRFQQQLE QLNAMGFLNR EANLQALIAT GGDINAAIER LLGSQPS.