Ubiquitin K48R Human
Ubiquitin K48R Human Recombinant
Cat. No.
BT22299
Source
Escherichia Coli.
Synonyms
Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin K48R.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Recombinant human ubiquitin featuring a Lys 48 to Arg48 mutation is useful for the reduction of poly-Ub chain length and conjugation rates.
Ubiquitin K48R is expressed in E.coliand purified by proprietary chromatographic techniques.
Ubiquitin K48R is expressed in E.coliand purified by proprietary chromatographic techniques.
Product Specs
Introduction
Ubiquitin, a highly conserved 76-amino acid protein found in all eukaryotes, exists in various forms: free, monomerically conjugated to proteins, or as ubiquitin chains. While polyubiquitination typically leads to substrate degradation, monoubiquitinated proteins remain stable. Ubiquitination involves a three-step process using a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3). The process begins with the transfer of a ubiquitin moiety to the ε-amino group of a lysine residue on the target protein, forming an isopeptide bond. Subsequent ubiquitin moieties are added to Lys48 of the previous ubiquitin, creating a polyubiquitin chain. Importantly, Ubiquitin K48R, with its Lys48 to Arg48 mutation, disrupts polyubiquitin chain formation via Lys48 linkages, limiting conjugation to mono-ubiquitination and preventing substrate degradation.
Description
Recombinant human ubiquitin K48R is designed to limit polyubiquitin chain formation and reduce conjugation rates. By substituting Lys48 with Arg48, this variant alters the ubiquitination process. Produced in E.coli and purified using proprietary chromatographic methods, it offers high purity for research applications.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The protein is supplied in a solution of PBS with 5% glycerol.
Stability
For long-term storage, keep the vial at a temperature between -20°C and -80°C. Under these conditions, the protein remains stable for up to 12 months. Repeated freezing and thawing should be avoided.
Purity
Analysis by SDS-PAGE confirms a purity exceeding 95%.
Synonyms
Ubiquitin, Ribosomal Protein S27a, CEP80, UBA80, UBCEP1, UBCEP80, HUBCEP80, RPS27A, Ubiquitin K48R.
Source
Escherichia Coli.
Product Science Overview
Introduction
Ubiquitin is a small regulatory protein that is ubiquitously expressed in eukaryotic cells. It plays a crucial role in various cellular processes, including protein degradation, cell cycle regulation, DNA repair, and response to oxidative stress. Ubiquitin achieves these functions through its ability to form polyubiquitin chains via lysine residues, with Lys48 (K48) being one of the most common linkage sites.
Ubiquitin K48R Mutation
Biological Properties
- Structure: Ubiquitin is a 76 amino acid protein with a highly conserved structure across different species. The K48R mutation does not significantly alter the overall structure of ubiquitin but specifically affects its ability to form K48-linked chains .
- Expression: Recombinant human ubiquitin K48R is typically expressed in E. coli systems, allowing for high-yield production and easy purification .
Functional Implications
- Protein Degradation: The K48R mutation inhibits the formation of K48-linked polyubiquitin chains, thereby preventing the ubiquitin-proteasome pathway from targeting proteins for degradation .
- Cellular Processes: By preventing K48-linked ubiquitination, the K48R mutant can be used to study the role of ubiquitin in various cellular processes, such as cell cycle regulation and DNA repair .
Applications in Research
- Protein Stability: Researchers use the K48R mutant to investigate the stability of proteins that are typically degraded via K48-linked ubiquitination. This helps in understanding the mechanisms of protein turnover and the role of ubiquitin in maintaining cellular homeostasis .
- Pathway Analysis: The K48R mutation is also used to dissect the ubiquitin-proteasome pathway and its involvement in diseases such as cancer and neurodegenerative disorders .
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