UBE2R2 Human

Ubiquitin Conjugating Enzyme E2R 2 Human Recombinant
Cat. No.
BT19386
Source
Escherichia Coli.
Synonyms
CDC34B, E2-CDC34B, UBC3B, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin-conjugating enzyme E2 R2, EC 6.3.2.19.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UBE2R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-238 a.a) and having a molecular mass of 29.6kDa (Molecular size on SDS-PAGE will appear higher).
UBE2R2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ubiquitin-conjugating enzyme E2 R2 (UBE2R2) belongs to the ubiquitin-conjugating enzyme family. Protein kinase CK2, a widespread and multifunctional Ser/Thr protein kinase, is involved in cell growth and transformation. UBE2R2 is similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme regulates β-TrCP substrate recognition, induces its interaction with β-TrCP, and enhances β-catenin degradation. UBE2R2 receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It exhibits in vitro activity in monoubiquitination and 'Lys-48'-linked polyubiquitination. UBE2R2 may be involved in katenin degradation. Notably, UBE2R2 is associated with diseases like cblc and herpes simplex.
Description
Recombinant human UBE2R2, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 261 amino acids (1-238 a.a) and has a molecular mass of 29.6kDa. (Note: On SDS-PAGE, the molecular size may appear higher). The protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile-filtered.
Formulation
The UBE2R2 protein solution (0.25mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 20% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 85.0% by SDS-PAGE analysis.
Synonyms
CDC34B, E2-CDC34B, UBC3B, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin-conjugating enzyme E2 R2, EC 6.3.2.19.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S

Product Science Overview

Introduction

Ubiquitin Conjugating Enzyme E2R 2 (UBE2R2), also known as UBC3B or CDC34B, is a member of the E2 ubiquitin-conjugating enzyme family. These enzymes play a crucial role in the ubiquitination process, which is essential for protein degradation and regulation within eukaryotic cells .

Structure and Function

UBE2R2 is a Ser/Thr protein kinase involved in cell growth and transformation. It is similar to the E2 ubiquitin-conjugating enzyme UBC3/CDC34 . The enzyme is responsible for transferring ubiquitin from the E1 activating enzyme to the E3 ligase, which then attaches ubiquitin to the target protein. This process marks the protein for degradation by the proteasome .

Recombinant UBE2R2

Recombinant UBE2R2 is typically produced in E. coli and is fused with an N-terminal His-tag for purification purposes. The recombinant protein corresponds to the amino acids 1-238 of the human UBE2R2 . It is used in various research applications to study protein ubiquitination and degradation pathways.

Biological Significance

UBE2R2 is involved in several cellular processes, including cell cycle regulation, DNA repair, and signal transduction. It has been shown to interact with beta-TrCP, enhancing beta-catenin degradation through CK2-dependent phosphorylation . This interaction is crucial for maintaining cellular homeostasis and preventing abnormal cell growth.

Applications in Research

Recombinant UBE2R2 is widely used in biochemical and cellular assays to investigate the mechanisms of ubiquitination and protein degradation. It is also employed in the development of targeted protein degradation technologies, such as Proteolysis-Targeting Chimeras (PROTACs), which aim to selectively degrade disease-related proteins .

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