UBE2M Human

UBE2M plays a crucial role in the process of neddylation, which involves the conjugation of the ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins . This process is essential for the regulation of various cellular functions, including morphogenesis and cell cycle progression .

The neddylation pathway requires the coordinated activities of UBE2M and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme . UBE2M receives NEDD8 from the E1 enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to target proteins . This modification modulates the levels of several key proteins, including p27/Kip1, beta-Catenin, and members of the Cullin family .

The NEDD8 pathway, mediated by UBE2M, is vital for the viability of many organisms and has significant implications in various biological processes . Dysregulation of this pathway can lead to various diseases, making UBE2M a potential target for therapeutic interventions .

Recombinant human UBE2M is produced using Escherichia coli (E. coli) expression systems . The recombinant protein is typically supplied as a solution in HEPES, NaCl, TCEP, and Glycerol, and is shipped with dry ice to maintain stability . It is recommended to store the protein at -70°C to ensure its longevity .

Recombinant UBE2M is used in various research applications to study the neddylation pathway and its role in cellular processes . It is also utilized in enzyme activity assays to understand the interaction between UBE2M and other proteins involved in the neddylation process .