UBE2D2 Human, His
Description
The UBE2D2 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM.
Product Science Overview
Ubiquitin Conjugating Enzyme E2D2, also known as UBE2D2, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme plays a crucial role in the ubiquitination process, which is essential for the regulated degradation of proteins within eukaryotic cells. The human recombinant form of UBE2D2, tagged with a His (histidine) tag, is commonly used in research to study its function and interactions.
Ubiquitination is a post-translational modification process that involves the attachment of ubiquitin, a small regulatory protein, to target proteins. This process is carried out through a cascade of enzymatic reactions involving three main types of enzymes:
- E1 Ubiquitin-Activating Enzyme: Activates ubiquitin in an ATP-dependent manner.
- E2 Ubiquitin-Conjugating Enzyme: Transfers the activated ubiquitin to the target protein.
- E3 Ubiquitin Ligase: Facilitates the transfer of ubiquitin from the E2 enzyme to the target protein, ensuring specificity.
UBE2D2 functions as an E2 enzyme, accepting ubiquitin from the E1 enzyme and catalyzing its covalent attachment to substrate proteins .
The human recombinant UBE2D2 is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain consisting of 167 amino acids. It has a molecular mass of approximately 18.9 kDa and includes a 20 amino acid His-tag at the N-terminus . The His-tag facilitates purification and detection of the protein using affinity chromatography techniques.
UBE2D2 is involved in the ubiquitination of several key proteins, including the tumor suppressor protein p53 and the peroxisomal biogenesis factor 5 (PEX5). It also plays a role in the ubiquitination of IκB-α in a SCFβ-TRCP and phosphorylation-dependent manner .
UBE2D2 is essential for maintaining cellular protein homeostasis by regulating the degradation of misfolded, damaged, or short-lived proteins via the ubiquitin-proteasome system (UPS). This system is critical for various cellular processes, including cell cycle regulation, DNA repair, and response to oxidative stress .
The human recombinant UBE2D2 with a His-tag is widely used in biochemical and structural studies to understand its enzymatic activity, substrate specificity, and interactions with other proteins. It is also utilized in high-throughput screening assays to identify potential inhibitors or modulators of the ubiquitination pathway, which could have therapeutic implications for diseases such as cancer and neurodegenerative disorders .
