UBA3 Human

Ubiquitin-Like Modifier Activating Enzyme 3 Human Recombinant
Cat. No.
BT17074
Source
Escherichia Coli.
Synonyms
NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C, hUBA3.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

UBA3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 487 amino acids (1-463) and having a molecular mass of 54.4kDa.
UBA3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The NEDD8-activating enzyme E1 catalytic subunit (UBA3) is a crucial component of the ubiquitin-like protein modification pathway. It functions as the catalytic subunit within the dimeric UBA3-NAE1 E1 enzyme, which plays a central role in activating NEDD8. The activation process involves a two-step mechanism: first, UBA3 adenylates the C-terminal glycine residue of NEDD8 using ATP, and then it links this residue to its catalytic cysteine, forming a NEDD8-UBA3 thioester and releasing AMP. Subsequently, UBA3 transfers NEDD8 to the catalytic cysteine of UBE2M, a NEDD8-conjugating enzyme. Notably, UBA3 interacts with AppBp1, an amyloid beta precursor protein-binding protein, to form a heterodimer that facilitates the activation of NEDD8. NEDD8, in turn, regulates essential cellular processes such as cell division, signaling, and embryogenesis.
Description
Recombinant human UBA3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 487 amino acids, including a 24 amino acid His-tag at the N-terminus, and has a molecular weight of 54.4 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The UBA3 solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the UBA3 solution should be kept at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing of the solution.
Purity
The purity of the UBA3 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3, UBA3, UBE1C, hUBA3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS.

Product Science Overview

Introduction

Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) is a crucial component in the ubiquitin-proteasome system, which is responsible for the degradation of proteins within the cell. This enzyme is part of the E1 ubiquitin-activating enzyme family and plays a significant role in the neddylation pathway, which is essential for various cellular processes, including cell division, signaling, and embryogenesis .

Gene and Protein Structure

UBA3 is encoded by the UBA3 gene located on chromosome 3p14.1 . The gene produces a protein that consists of 442 amino acids and shares 43% sequence identity with its yeast homolog . The UBA3 protein forms a heterodimer with APPBP1 (amyloid beta precursor protein binding protein 1), which is necessary for its function .

Function and Mechanism

UBA3, in conjunction with APPBP1, forms the NEDD8-activating enzyme (NAE). This enzyme complex is responsible for the activation of NEDD8, a ubiquitin-like protein. The activation process involves the adenylation of the C-terminal glycine residue of NEDD8 with ATP, followed by the formation of a thioester bond between NEDD8 and the catalytic cysteine residue of UBA3 . This activated NEDD8 is then transferred to specific target proteins, regulating their stability and function.

Biological Significance

The neddylation pathway, mediated by UBA3, is crucial for the regulation of the cullin-RING ubiquitin ligases (CRLs). These ligases are involved in the ubiquitination and subsequent degradation of various proteins, thereby controlling numerous cellular processes . Dysregulation of this pathway can lead to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.

Research and Applications

Research on UBA3 has provided insights into its role in cellular homeostasis and disease. The recombinant form of UBA3 is used in various studies to understand its function and to develop potential therapeutic interventions. For instance, inhibitors targeting the NEDD8-activating enzyme are being explored as potential treatments for cancer, as they can disrupt the degradation of proteins that promote tumor growth .

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