U2AF1 Human

U2 Small Nuclear RNA Auxiliary Factor 1 Human Recombinant
Cat. No.
BT27952
Source
Escherichia Coli.
Synonyms
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF35, U2AFBP, FP793, RNU2AF1, RN.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

U2AF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-240 a.a) and having a molecular mass of 30.3kDa.
U2AF1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
U2AF1 is a member of the SR family of splicing factors. It is a subunit of the U2 auxiliary factor, a protein composed of a large and a small subunit. This factor is a non-snRNP protein that is essential for the binding of U2 snRNP to the pre-mRNA branch site. U2AF1 is the small subunit of this factor and plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly facilitating interactions between the large subunit and proteins bound to the enhancers. This protein is encoded by the U2AF1 gene. Among the diseases associated with U2AF1 are myoclonus epilepsy and progressive myoclonus epilepsy.
Description
Recombinant human U2AF1 was produced in E. coli. It is a single, non-glycosylated polypeptide chain containing 263 amino acids (amino acids 1-240). This protein has a molecular mass of 30.3 kDa. A 23 amino acid His-tag is fused to the N-terminus of U2AF1. The protein was purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The U2AF1 protein solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For longer-term storage, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 90% purity as determined by SDS-PAGE.
Synonyms
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF35, U2AFBP, FP793, RNU2AF1, RN.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF

Product Science Overview

Introduction

U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) is a crucial component of the spliceosome, a complex responsible for the removal of introns from pre-mRNA. This protein plays a significant role in RNA splicing, a process essential for the proper expression of genes. U2AF1 is a non-snRNP (small nuclear ribonucleoprotein) protein that is required for the binding of U2 snRNP to the pre-mRNA branch site .

Structure and Function

U2AF1 is a subunit of the U2 Auxiliary Factor complex, which also includes a larger subunit known as U2AF2. The U2AF1 protein is approximately 35 kDa in size and is encoded by the U2AF1 gene located on chromosome 21q22.3 . The primary function of U2AF1 is to recognize and bind to AG nucleotides at the 3’ splice site, facilitating the assembly of the spliceosome .

Role in RNA Splicing

RNA splicing is a critical step in the post-transcriptional modification of RNA. U2AF1, along with U2AF2, ensures the accurate removal of introns and the joining of exons to form mature mRNA. This process is vital for the generation of functional proteins. U2AF1 directly mediates interactions between the large subunit (U2AF2) and proteins bound to enhancers, playing a critical role in both constitutive and enhancer-dependent RNA splicing .

Clinical Significance

Mutations in the U2AF1 gene have been associated with various hematologic malignancies, particularly myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) . These mutations often occur at two hotspot locations, S34 and Q157, within the zinc-finger domains of the U2AF1 protein . Such mutations can lead to altered splicing patterns, contributing to the pathogenesis of these diseases .

Research and Applications

Recombinant U2AF1 protein is used in various research applications to study its role in RNA splicing and its implications in disease. Understanding the function and regulation of U2AF1 can provide insights into the mechanisms of splicing-related disorders and potentially lead to the development of targeted therapies .

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