TYMS Antibody

Thymidylate synthetase (TS) is a crucial enzyme in the biosynthesis of DNA. It catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), a necessary step for the formation of thymine nucleotides. This process is essential for DNA replication and repair, making TS a vital enzyme in proliferating cells .

Thymidylate synthetase is a homodimer composed of two 30 kDa subunits. The enzyme’s activity is highest in cells that are actively dividing, as it provides the sole de novo source of thymidylate. The methylation reaction it catalyzes uses 10-methylenetetrahydrofolate (methylene-THF) as a cofactor .

TS is an important target for anticancer agents, such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is effective against various solid tumors, including those in the colon, breast, head, and neck. Research has shown that patients with tumors expressing lower levels of TS have a higher response rate to 5-FU treatment compared to those with higher TS levels .

Mouse anti-human thymidylate synthetase antibodies are monoclonal antibodies produced by immunizing mice with recombinant human TS protein. These antibodies are highly specific and are used in various applications, including Western blotting (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) .

1. Western Blotting (WB): These antibodies are used to detect endogenous levels of TS in various cell lines. They are typically diluted at a ratio of 1:1000 for this application .
2. Immunohistochemistry (IHC): In IHC, these antibodies are used to visualize TS expression in tissue samples. The recommended dilution for IHC is 1:100 to 1:200 .
3. Enzyme-Linked Immunosorbent Assay (ELISA): These antibodies can also be used in ELISA to quantify TS levels in different samples .

Mouse anti-human thymidylate synthetase antibodies are typically supplied in a buffer containing sodium HEPES, NaCl, BSA, glycerol, and sodium azide. They should be stored at -20°C and should not be aliquoted to maintain their stability .