TXNL4B Human
Thioredoxin-like protein 4B, Dim1-like protein, TXNL4B, DIM2, DLP.
Description
TXNL4B produced in E.Coli is a single, non-glycosylated polypeptide chain containing 185 amino acids (1-149 a.a) and having a molecular mass of 21.1kDa.
TXNL4B is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Thioredoxin-like protein 4B, Dim1-like protein, TXNL4B, DIM2, DLP.
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSFL LPKLTSKKEV DQAIKSTAEK VLVLRFGRDE DPVCLQLDDI LSKTSSDLSK MAAIYLVDVD QTAVYTQYFD ISYIPSTVFF FNGQHMKVDY GSPDHTKFVG SFKTKQDFID LIEVIYRGAM RGKLIVQSPI DPKNIPKYDL LYQDI.
Product Science Overview
The TXNL4B gene is located on chromosome 16 and is a protein-coding gene . The gene produces a protein that consists of 185 amino acids and has a molecular mass of approximately 21.1 kDa . The recombinant form of TXNL4B is typically produced in Escherichia coli and is a single, non-glycosylated polypeptide chain . It is often fused to a His-tag at the N-terminus to facilitate purification .
TXNL4B is essential for pre-mRNA splicing, a critical process in gene expression where introns are removed, and exons are joined to form mature mRNA . This protein interacts with the PRPF6 subunit of the spliceosome, a complex responsible for splicing . Additionally, TXNL4B is required for cell cycle progression, particularly during the transition from the S phase to the G2 phase .
Mutations or dysregulation of the TXNL4B gene have been associated with several diseases. Notably, it is linked to Optic Atrophy 7, which may or may not be accompanied by auditory neuropathy . Another associated condition is Fanconi Anemia, Complementation Group A, a genetic disorder that affects bone marrow and results in decreased production of all types of blood cells .
The recombinant form of TXNL4B is produced using E. coli expression systems . This method involves cloning the TXNL4B gene into a plasmid vector, which is then introduced into E. coli cells. The bacteria express the protein, which is subsequently purified using chromatographic techniques . The recombinant protein is used in various research applications, including studies on mRNA splicing, cell cycle regulation, and disease mechanisms.
The recombinant TXNL4B protein is typically stored in a solution containing Tris-HCl buffer, DTT, glycerol, and NaCl . It is stable at 4°C for up to one week but should be stored below -18°C for long-term preservation . It is important to avoid repeated freeze-thaw cycles to maintain protein integrity .
