TXNL4A Human

Thioredoxin-Like 4A Human Recombinant
Cat. No.
BT13031
Source
E.coli.
Synonyms
Thioredoxin-like 4A, Spliceosomal U5 snRNP-specific 15 kDa protein, DIM1 protein homolog, HsT161, TXNL4, DIB1, U5-15kD.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TXNL4A Human Recombinant produced in E. coli is a single polypeptide chain containing 166 amino acids (1-142) and having a molecular mass of 19.3kDa.
TXNL4A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TXNL4A, a member of the Dim protein family, plays a crucial role in pre-mRNA splicing. In Schizosaccharomyces pombe, the absence of the TXNL4A gene leads to embryonic death during gastrulation due to the inability to express early zygotic transcripts. TXNL4A is localized in the nucleus and interacts with hnRNP F, hnRNP H2, Cas-L, and PQBP-1 to regulate gene expression.
Description
Recombinant human TXNL4A, produced in E. coli, is a single polypeptide chain consisting of 166 amino acids (residues 1-142) with a molecular weight of 19.3 kDa. This protein includes a 24 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The TXNL4A solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 200 mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For longer storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95% as determined by SDS-PAGE analysis.
Synonyms
Thioredoxin-like 4A, Spliceosomal U5 snRNP-specific 15 kDa protein, DIM1 protein homolog, HsT161, TXNL4, DIB1, U5-15kD.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSYMLP HLHNGWQVDQ AILSEEDRVV VIRFGHDWDP TCMKMDEVLY SIAEKVKNFA VIYLVDITEV PDFNKMYELY DPCTVMFFFR NKHIMIDLGT GNNNKINWAM EDKQEMVDII ETVYRGARKG RGLVVSPKDY STKYRY

Product Science Overview

Structure and Function

TXNL4A contains a thioredoxin-like fold, which is a characteristic structural motif found in thioredoxin proteins . This fold allows TXNL4A to interact with multiple proteins, including the polyglutamine tract-binding protein 1 (PQBP1) . The primary function of TXNL4A is to participate in the assembly of the spliceosome, a complex responsible for removing introns from pre-mRNA .

Biological Role

TXNL4A plays a crucial role in the processing of capped intron-containing pre-mRNA and is involved in the mRNA splicing minor pathway . It is a component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes, which are essential for the formation of the spliceosome . The protein’s interactions and functions are vital for the accurate and efficient splicing of pre-mRNA, ensuring proper gene expression and cellular function .

Clinical Significance

Mutations in the TXNL4A gene have been associated with Burn-McKeown syndrome, a rare genetic disorder characterized by craniofacial dysmorphisms, cardiac defects, hearing loss, and bilateral choanal atresia . Additionally, TXNL4A is linked to Developmental and Epileptic Encephalopathy 7, highlighting its importance in neurological development and function .

Research and Applications

Recombinant forms of TXNL4A are used in research to study its structure, function, and interactions with other proteins. Understanding the role of TXNL4A in pre-mRNA splicing and its involvement in genetic disorders can provide insights into potential therapeutic targets for related diseases.

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THE BIOTEK
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