TXNL1 Human

Thioredoxin-Like 1 Human Recombinant
Cat. No.
BT12967
Source
Escherichia Coli.
Synonyms
Thioredoxin-like protein 1, TRP32, Txl, 32 kDa thioredoxin-related protein, TXNL, Thioredoxin-like 32kDa, TXL-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TXNL1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 309 amino acids (1-289.a.a) and having a molecular mass of 34.4kDa.
TXNL1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TXNL1, found in the cytoplasm, plays a crucial role in endocytosis signaling and acts as a redox sensor. This protein is expressed throughout the body and works to link oxidative stress with endocytosis, effectively regulating the GDI. Furthermore, elevated levels of TXNL1 have been shown to hinder cell proliferation by pushing cells into the G0/G1 arrest phase, suggesting that TXNL1 may also act as a transcriptional repressor.
Description
Produced in E. coli, TXNL1 is a single, non-glycosylated polypeptide chain comprising 309 amino acids (specifically, amino acids 1 through 289). It possesses a molecular weight of 34.4 kDa. For purification purposes, a 20 amino acid His-tag is fused to the N-terminus of the protein, and proprietary chromatographic techniques are employed.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The TXNL1 protein solution is provided at a concentration of 0.5 mg/ml. The solution is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 5 mM DTT, and 20% glycerol.
Purity
The purity level exceeds 95% as determined by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, store the product frozen at -20°C. Repeated freezing and thawing cycles should be avoided.
Synonyms
Thioredoxin-like protein 1, TRP32, Txl, 32 kDa thioredoxin-related protein, TXNL, Thioredoxin-like 32kDa, TXL-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MVGVKPVGSD PDFQPELSGA GSRLAVVKFT MRGCGPCLRI APAFSSMSNK YPQAVFLEVD VHQCQGTAAT NNISATPTFL FFRNKVRIDQ YQGADAVGLE EKIKQHLEND PGSNEDTDIP KGYMDLMPFI NKAGCECLNE SDEHGFDNCL RKDTTFLESD CDEQLLITVA FNQPVKLYSM KFQGPDNGQG PKYVKIFINL PRSMDFEEAE RSEPTQALEL TEDDIKEDGI VPLRYVKFQN VNSVTIFVQS NQGEEETTRI SYFTFIGTPV QATNMNDFKR VVGKKGESH

Product Science Overview

Introduction

Thioredoxin-Like 1 (TXNL1), also known as Thioredoxin-Related Protein 32 (TRP32), is a protein encoded by the TXNL1 gene in humans. This protein is part of the thioredoxin family, which plays a crucial role in maintaining cellular redox homeostasis. TXNL1 is involved in various cellular processes, including DNA synthesis, cell proliferation, and apoptosis.

Discovery and Structure

Thioredoxin-Like 1 was first identified as a protein with disulfide oxidoreductase activity, similar to other members of the thioredoxin family . The protein contains a redox-active site with two cysteine residues, which are essential for its reducing activity. These cysteines form a disulfide bond that can be reduced to free thiols, allowing TXNL1 to participate in redox reactions.

Function and Mechanism

TXNL1 functions primarily as a disulfide oxidoreductase, catalyzing the reduction of disulfide bonds in substrate proteins. This activity is crucial for maintaining the redox state of the cell and protecting against oxidative stress . TXNL1 is also involved in the regulation of various cellular processes, including:

  • DNA Synthesis: TXNL1 provides reducing equivalents for ribonucleotide reductase, an enzyme essential for DNA synthesis .
  • Cell Proliferation: By maintaining the redox state of key signaling molecules, TXNL1 supports cell growth and division .
  • Apoptosis: TXNL1 can modulate the activity of apoptosis-related proteins, thereby influencing cell survival and death .
Clinical Significance

Mutations or dysregulation of the TXNL1 gene have been associated with several diseases, including immunodeficiency and certain types of cancer . The protein’s role in redox regulation makes it a potential target for therapeutic interventions aimed at modulating oxidative stress and related pathologies.

Research and Applications

Recombinant human TXNL1 is widely used in research to study its function and potential therapeutic applications. By expressing and purifying the protein in a controlled environment, researchers can investigate its biochemical properties and interactions with other cellular components .

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