TXNDC17 Human
Description
TXNDC17 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
TXNDC17 is characterized by its thioredoxin fold and a thioredoxin-like active-site sequence . The primary function of thioredoxins, including TXNDC17, is to reduce protein disulfide bonds and participate in denitrosylation of nitrosylated proteins and thiols . This reduction process is essential for maintaining cellular redox homeostasis and protecting cells from oxidative stress.
The active site of TXNDC17 contains a dithiol in a CXXC motif, which is critical for its ability to reduce other proteins . The reduction process begins with the attack of one cysteine residue on the oxidized group of the substrate, followed by the formation of a disulfide bond with the second cysteine residue, thereby transferring electrons to the substrate and reducing it .
TXNDC17 is involved in various cellular processes, including the modulation of tumor necrosis factor-alpha (TNF-alpha) signaling and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa-B) activation . It also exhibits peroxidase activity, contributing to the elimination of cellular hydrogen peroxide . These functions highlight the importance of TXNDC17 in cellular defense mechanisms against oxidative damage.
Mutations or dysregulation of TXNDC17 have been associated with certain diseases. For instance, TXNDC17 has been linked to Miliaria Crystallina, a skin condition characterized by the blockage of sweat ducts . Additionally, the protein’s role in redox regulation and signaling pathways suggests its potential involvement in various pathological conditions, including cancer and inflammatory diseases.
Human recombinant TXNDC17 is produced using recombinant DNA technology, which involves the insertion of the TXNDC17 gene into an expression vector, followed by the transformation of a suitable host organism, such as Escherichia coli. The host organism then expresses the protein, which can be purified using various chromatographic techniques. Recombinant production allows for the generation of large quantities of TXNDC17 for research and therapeutic purposes.
