TXNDC12 Human

Thioredoxin Domain Containing 12 Human Recombinant
Cat. No.
BT12822
Source
E.coli.
Synonyms
Thioredoxin domain containing 12 (endoplasmic reticulum), Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, endoplasmic reticulum thioredoxin superfamily member 18 kDa, thioredoxin domain-containing protein 12, protein disulfide isomerase family A member 16, endoplasmic reticulum protein ERp19, anterior gradient homolog 1, Thioredoxin-like protein p19, ER protein 18, ERP18, ER protein 19, ERP19, ERP16, TLP19, AGR1, PDIA16, hAG-1, EC 1.8.4.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TXNDC12 Human Recombinant produced in E. coli is a single polypeptide chain containing 184 amino acids (27-172) and having a molecular mass of 20.8 kDa.
TXNDC12 is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TXNDC12, a member of the thioredoxin superfamily, plays a crucial role in cellular processes such as redox regulation, oxidative stress response, and transcription factor regulation. These proteins share a conserved thioredoxin fold characterized by a CxxC active site sequence.
Description
Recombinant TXNDC12, expressed in E. coli, is a single polypeptide chain consisting of 184 amino acids (residues 27-172) with a molecular weight of 20.8 kDa. The protein is purified using proprietary chromatographic techniques and features an N-terminal 38 amino acid His-tag.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The TXNDC12 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for prolonged storage. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Thioredoxin domain containing 12 (endoplasmic reticulum), Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, endoplasmic reticulum thioredoxin superfamily member 18 kDa, thioredoxin domain-containing protein 12, protein disulfide isomerase family A member 16, endoplasmic reticulum protein ERp19, anterior gradient homolog 1, Thioredoxin-like protein p19, ER protein 18, ERP18, ER protein 19, ERP19, ERP16, TLP19, AGR1, PDIA16, hAG-1, EC 1.8.4.2.
Source
E.coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMHN GLGKGFGDHI HWRTLEDGKK EAAASGLPLM VIIHKSWCGA CKALKPKFAE STEISELSHN FVMVNLEDEE EPKDEDFSPD GGYIPRILFL DPSGKVHPEI INENGNPSYK YFYVSAEQVV QGMKEAQERL TGDAFRKKHL EDEL

Product Science Overview

Introduction

Thioredoxin Domain Containing 12 (TXNDC12) is a member of the thioredoxin family, which plays a crucial role in maintaining cellular redox homeostasis. Thioredoxins are small redox proteins that are present in all organisms and are involved in various biological processes, including redox signaling, cell proliferation, and apoptosis .

Structure and Function

TXNDC12, like other thioredoxins, contains a characteristic thioredoxin fold and an active site with a conserved CXXC motif. This motif is essential for the protein’s oxidoreductase activity, allowing it to reduce disulfide bonds in other proteins . The reduction of disulfide bonds is a critical process in maintaining protein structure and function, as well as in protecting cells from oxidative stress.

Role in Cellular Processes

Thioredoxins, including TXNDC12, are involved in various cellular processes. They act as electron donors to peroxidases and ribonucleotide reductase, playing a significant role in DNA synthesis and repair . Additionally, thioredoxins are involved in the regulation of transcription factors and the activation of signaling pathways that control cell growth and survival .

Thioredoxin System

The thioredoxin system consists of thioredoxin, thioredoxin reductase, and NADPH. Thioredoxin reductase is responsible for maintaining thioredoxin in its reduced state, which is necessary for its oxidoreductase activity . This system is crucial for protecting cells from oxidative damage and maintaining redox balance.

Human Recombinant TXNDC12

Human recombinant TXNDC12 is produced using recombinant DNA technology, which involves inserting the gene encoding TXNDC12 into a suitable expression system, such as bacteria or yeast. This allows for the large-scale production of the protein, which can be used for research and therapeutic purposes.

Applications in Research and Medicine

Human recombinant TXNDC12 is used in various research applications to study its role in cellular processes and its potential therapeutic benefits. For example, it is used to investigate the mechanisms of redox regulation and the development of diseases associated with oxidative stress, such as cancer and neurodegenerative disorders . Additionally, TXNDC12 and other thioredoxins are being explored as potential targets for drug development, aiming to modulate their activity to treat various diseases .

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