T.Vaginalis P65 Antibody

The Polyclonal Rabbit Anti Trichomonas Vaginalis P65 Antibody is a specialized antibody used in research to detect the presence of the P65 protein, an adhesion protein of the protozoan parasite Trichomonas vaginalis. This parasite is the causative agent of trichomoniasis, a common sexually transmitted infection (STI) in humans .

Trichomonas vaginalis is an anaerobic, flagellated protozoan parasite that infects the urogenital tract of humans. The infection process involves multiple steps, including the interaction between host macromolecules and protozoan virulent factors. One of the key virulent factors is the P65 adhesion protein, which plays a crucial role in the parasite’s ability to adhere to and penetrate the vaginal tract surface .

The production of the polyclonal antibody involves immunizing rabbits with recombinant P65 protein from Trichomonas vaginalis. The rabbits’ immune systems produce antibodies against the P65 protein, which are then collected and purified using affinity chromatographic techniques. The resulting antibody is a polyclonal antibody, meaning it consists of a mixture of antibodies that recognize multiple epitopes on the P65 protein .

The P65 protein is a prominent adhesin that mediates the binding of Trichomonas vaginalis to host vaginal epithelial cells (VECs). The binding domain of the P65 protein interacts with both the parasite and the host cells, facilitating the adhesion process. This interaction is crucial for the parasite’s ability to establish infection and cause disease .

The Polyclonal Rabbit Anti Trichomonas Vaginalis P65 Antibody is used in various scientific applications, including:

• Enzyme-Linked Immunosorbent Assay (ELISA): To detect the presence of P65 protein in samples.
• Immunocytochemistry (ICC/IF): To visualize the localization of P65 protein in cells.
• Western Blotting (WB): To identify and quantify the P65 protein in different samples .

The antibody is typically supplied in a sterile, filtered, clear colorless solution. For short-term storage (2-4 weeks), it should be kept at 4°C. For long-term storage, it is recommended to freeze the antibody at -20°C, with the addition of a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA) to prevent degradation. Multiple freeze-thaw cycles should be avoided to maintain the antibody’s integrity .