TUBB3 Antibody
Description
Product Specs
Product Science Overview
Tubulin beta-3, also known as Class III beta-tubulin or beta-III tubulin, is a microtubule element of the tubulin family. It is predominantly found in neurons and testis cells . This protein is encoded by the TUBB3 gene in humans . Tubulin beta-3 plays a crucial role in the formation and stabilization of microtubules, which are essential components of the cytoskeleton. Microtubules are involved in various cellular processes, including cell division, intracellular transport, and maintenance of cell shape .
In neurons, tubulin beta-3 is particularly important for neurogenesis, axon guidance, and maintenance . It is conditionally expressed in other tissues under certain conditions, such as exposure to a toxic microenvironment characterized by hypoxia and poor nutrient supply . Posttranslational modifications, including phosphorylation and glycosylation, are required for its functional activity .
Mutations in the TUBB3 gene can lead to various neurological disorders, including congenital fibrosis of the extraocular muscles type 3 . This condition is characterized by restricted eye movements and other ocular abnormalities.
Mouse anti-human antibodies are secondary antibodies that are generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins. They are commonly used in various immunological assays, including enzyme-linked immunosorbent assay (ELISA), Western blotting, flow cytometry, and immunohistochemistry .
Secondary antibodies, such as mouse anti-human antibodies, offer increased versatility and sensitivity in detection systems. They can bind to multiple primary antibodies, allowing for signal amplification and improved detection of target antigens . These antibodies are often conjugated with various labels, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), or fluorescent dyes, to facilitate detection .
Mouse anti-human antibodies are widely used in research and diagnostic applications to detect and quantify human proteins, study protein-protein interactions, and investigate cellular processes . They are essential tools in the fields of immunology, cell biology, and molecular biology.
The combination of tubulin beta-3 and mouse anti-human antibodies is particularly valuable in neuroscience research. Tubulin beta-3 serves as a marker for mature neurons, allowing researchers to identify and study neuronal populations in brain tissue samples . By using mouse anti-human antibodies, scientists can specifically detect human proteins in various experimental settings, enabling the investigation of human-specific cellular processes and disease mechanisms .
