TUBB2B Antibody
Description
Product Specs
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
Tubulin beta-2B chain, also known as TUBB2B, is a protein that plays a crucial role in the formation and function of microtubules. Microtubules are essential components of the cytoskeleton, providing structural support and facilitating intracellular transport, cell division, and other critical cellular processes.
Tubulin exists as a heterodimer composed of alpha and beta subunits. The beta-2B chain is one of the several beta-tubulin isotypes. It is predominantly expressed in neurons but is also found in other tissues such as lung tissue and Schwann cells . The beta-2B chain is involved in the polymerization of tubulin into microtubules, which are dynamic structures that undergo rapid assembly and disassembly.
TUBB2B has been implicated in various diseases, particularly cancers. Increased expression of TUBB2B has been observed in hepatocellular carcinomas, indicating its potential role in tumorigenesis . Additionally, mutations in the TUBB2B gene have been associated with brain malformations, such as polymicrogyria, which is characterized by abnormal cortical development.
The mouse anti-human TUBB2B antibody is a monoclonal antibody developed to specifically target the TUBB2B protein in human samples. This antibody is derived from the hybridization of mouse myeloma cells with spleen cells from mice immunized with recombinant human TUBB2B . It is commonly used in various scientific applications, including Western Blot, Immunocytochemistry, and Immunohistochemistry .
- Western Blot: The mouse anti-human TUBB2B antibody is used to detect the presence of TUBB2B protein in cell extracts. It is typically used at a concentration of 0.25 - 1.0 µg/mL .
- Immunohistochemistry: This antibody is used to visualize TUBB2B expression in formalin-fixed paraffin-embedded tissue sections. A concentration range of 1.0 - 10.0 µg/mL is recommended .
- Immunocytochemistry: The antibody is also used to study TUBB2B expression in cultured cells. A concentration range of 2.5 - 10.0 µg/mL is suggested .
