TSTD3 Human

The TSTD3 gene is located on chromosome 6 and encodes a protein that is 120 amino acids long with a molecular mass of approximately 13.7 kDa . The protein is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain . The recombinant form of this protein is often fused with a His-tag at the N-terminus to facilitate purification through chromatographic techniques .

The primary function of TSTD3 is to facilitate the transfer of sulfur atoms within the cell. This is crucial for various biochemical pathways, including the detoxification of cyanide and the biosynthesis of iron-sulfur clusters . The active site cysteine residue in the rhodanese domain plays a pivotal role in these sulfur transfer reactions .

Mutations or dysregulation of the TSTD3 gene have been linked to certain diseases. For instance, TSTD3 has been associated with Leber Congenital Amaurosis 19, a genetic disorder that leads to severe vision loss at an early age . Understanding the function and regulation of TSTD3 can provide insights into the pathogenesis of such diseases and potentially lead to the development of targeted therapies.

Recombinant TSTD3 is widely used in laboratory research to study sulfur transfer reactions and their implications in various biological processes. The protein is available in different quantities and purities for research purposes . It is typically stored in a Tris-HCl buffer solution with glycerol and DTT to maintain stability .