TSNAX Human
Translin-Associated Factor X Human Recombinant
Cat. No.
BT27091
Source
Escherichia Coli.
Synonyms
TRAX, Translin-associated protein X, Translin-associated factor X.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TSNAX Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-290 a.a.) and having a molecular mass of 35kDa.
TSNAX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TSNAX is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
TSNAX, part of the translin protein family, interacts with translin, a DNA-binding protein. This interaction is notable as translin binds to specific DNA sequences found at chromosomal translocation breakpoints. TSNAX and TSN work together as an endonuclease, playing a role in activating the RNA-induced silencing complex (RISC). Notably, TSNAX possesses bipartite nuclear targeting sequences that facilitate the transport of translin, which lacks these sequences, into the nucleus.
Description
Recombinant human TSNAX, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein comprises 313 amino acids (specifically, amino acids 1 to 290) and has a molecular weight of 35 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of TSNAX. The purification process utilizes proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterilization by filtration.
Formulation
The TSNAX protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 50% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. If longer storage is required, freezing at -20°C is recommended. The addition of a carrier protein, such as 0.1% HSA or BSA, is advisable for extended storage. It is crucial to avoid repeated cycles of freezing and thawing.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Synonyms
TRAX, Translin-associated protein X, Translin-associated factor X.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSNKEGS GGFRKRKHDN FPHNQRREGK DVNSSSPVML AFKSFQQELD ARHDKYERLV KLSRDITVES KRTIFLLHRI TSAPDMEDIL TESEIKLDGV RQKIFQVAQE LSGEDMHQFH RAITTGLQEY VEAVSFQHFI KTRSLISMDE INKQLIFTTE DNGKENKTPS SDAQDKQFGT WRLRVTPVDY LLGVADLTGE LMRMCINSVG NGDIDTPFEV SQFLRQVYDG FSFIGNTGPY EVSKKLYTLK QSLAKVENAC YALKVRGSEI PKHMLADVFS VKTEMIDQEE GIS.
Product Science Overview
Gene and Protein Structure
Function and Biological Role
TSNAX plays a crucial role in various cellular processes. It forms a heteromeric complex with translin, known as the C3PO complex (Component 3 Promoter of RISC), which is involved in the RNA-induced silencing complex (RISC) pathway . This complex facilitates the endonucleolytic cleavage of the passenger strand during microRNA loading into RISC, a critical step in RNA interference .
Additionally, TSNAX has been implicated in several biological processes, including:
Expression and Localization
Interactions and Complex Formation
Clinical Significance
Research and Applications
The recombinant form of TSNAX is used in research to study its role in RNA interference and other cellular processes. Understanding the function and regulation of TSNAX can provide insights into its involvement in disease mechanisms and potential therapeutic targets.
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