TSN Human
Translin Human Recombinant
Cat. No.
BT27014
Source
Escherichia Coli.
Synonyms
Translin, TRSLN, BCLF-1, REHF-1, RCHF1, TBRBP, Recombination Hotspot-binding Protein, Recombination Hotspot Associated factor.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TSN produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (1-228a.a.) and having a molecular mass of 26.1kDa.
TSN is purified by proprietary chromatographic techniques.
TSN is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Translin, also known as TSN, is a protein that plays a crucial role in binding DNA and RNA. It specifically recognizes and attaches to conserved target sequences found at the points where chromosomes break and rejoin, known as chromosomal translocations. TSN forms a ring-like structure, which is essential for its DNA binding activity. Additionally, it possesses a leucine zipper motif, thought to facilitate the formation of TSN dimers (pairs of TSN molecules). TSN is involved in transporting specific messenger RNA (mRNA) molecules out of the nucleus, the cell's control center. This function is supported by its presence in both the nucleus and cytoplasm of neurons. Moreover, TSN regulates the translation of these mRNAs, influencing protein synthesis.
Description
TSN, produced in E. coli bacteria, is a single polypeptide chain that lacks any glycosylation modifications. It comprises 228 amino acids (specifically, amino acids 1 to 228) and has a molecular weight of 26.1 kilodaltons (kDa). The purification of TSN is achieved using specialized chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The TSN protein solution (containing 1 mg of TSN per 1 ml of solution) is prepared in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 100 mM NaCl, and 10% glycerol.
Stability
For optimal storage, keep the TSN protein at 4°C if you plan to use the entire vial within 2 to 4 weeks. If you need to store it for a longer duration, freeze the solution at -20°C. For extended storage periods, it is advisable to add a carrier protein (0.1% HSA or BSA) to protect the TSN protein. Avoid repeated cycles of freezing and thawing.
Purity
The purity of the TSN protein is greater than 90%, as assessed by SDS-PAGE analysis.
Synonyms
Translin, TRSLN, BCLF-1, REHF-1, RCHF1, TBRBP, Recombination Hotspot-binding Protein, Recombination Hotspot Associated factor.
Source
Escherichia Coli.
Amino Acid Sequence
MSVSEIFVEL QGFLAAEQDI REEIRKVVQS LEQTAREILT LLQGVHQGAG FQDIPKRCLK AREHFGTVKT HLTSLKTKFP AEQYYRFHEH WRFVLQRLVF LAAFVVYLET ETLVTREAVT EILGIEPDRE KGFHLDVEDY LSGVLILASE LSRLSVNSVT AGDYSRPLHI STFINELDSG FRLLNLKNDS LRKRYDGLKY DVKKVEEVVY DLSIRGFNKE TAAACVEK.
Product Science Overview
Structure and Function
Translin forms an octameric ring structure, which is crucial for its function . This protein is primarily cytoplasmic but migrates to the nucleus under genotoxic stress . It binds to breakpoint hotspots, implicating its role in chromosomal translocation events and DNA damage response .
Translin’s DNA-binding activity is facilitated by its multimeric structure, which recognizes conserved target sequences at chromosomal translocation junctions . These binding activities are significant in chromosomal translocations in lymphoid neoplasms .
Biochemical Activities
Translin is involved in various biochemical activities, including:
- DNA recombination: Recognizes and binds to recombination-associated motifs at recombination hotspots .
- RNA metabolism: Plays a role in tRNA processing, RNA interference (RNAi), RNA transport, and mRNA transport .
- RNA silencing: Forms a heteromeric complex with Translin-associated factor X (TRAX) to create the C3PO complex, which is involved in RNA silencing .
Cellular Functions
Translin has several cellular functions:
- DNA Damage Response: Migrates to the nucleus under genotoxic stress and binds to DNA breakpoints .
- Chromosomal Translocation: Involved in chromosomal translocation events, particularly in lymphoid neoplasms .
- RNA Transport and Cell Cycle Progression: Binds to 3′ and 5′ UTR of certain mRNAs, regulating their availability for translation .
Clinical Implications
Translin’s role in DNA damage repair and chromosomal translocation makes it a significant protein in understanding certain malignancies and genetic disorders . Its involvement in RNA metabolism and RNA silencing also highlights its potential as a target for therapeutic interventions in diseases related to RNA processing and regulation .
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