TSG101 Human
Tumor Susceptibility Gene 101 Human Recombinant
Cat. No.
BT26963
Source
Escherichia Coli.
Synonyms
TSG10, VPS23, TSG101, ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TSG101 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 181 amino acids (1-145 a.a.) and having a molecular mass of 20.7 kDa. TSG101 protein is fused to a 36 amino acid His-Tag at N-terminus and purified by standard chromatography.
Product Specs
Introduction
TSG101, an inactive homolog of ubiquitin-conjugating enzymes, plays a crucial role in cell growth, differentiation, and genomic stability. It interacts with stathmin, a protein linked to tumorigenesis, and acts as a negative growth regulator. Maintaining stable TSG101 expression is vital for proper cell cycle regulation and preventing genomic instability. Mutations and alternative splicing of TSG101 are frequent in breast cancer, suggesting its involvement in tumor development and progression. As a component of the ESCRT-I complex, TSG101 participates in vesicular trafficking by binding ubiquitinated cargo proteins and directing them into multivesicular bodies. This process is essential for completing cytokinesis and regulating cell growth and differentiation.
Description
Recombinant human TSG101, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 181 amino acids (residues 1-145) with a molecular weight of 20.7 kDa. This protein includes an N-terminal 36-amino acid His-Tag and undergoes purification using standard chromatography techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The TSG101 protein solution is provided at a concentration of 0.5 mg/ml and is buffered with 20 mM Tris-HCl (pH 8), 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of the TSG101 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
TSG10, VPS23, TSG101, ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P.
Product Science Overview
Structure and Function
The TSG101 protein contains several unique domains, including:
- Inactive ubiquitin-conjugating domain: Despite being homologous to ubiquitin-conjugating enzymes, this domain is inactive.
- Proline-rich region: This region is involved in protein-protein interactions.
- Coiled-coil domain: Facilitates the formation of protein complexes.
- Steadiness box: Important for the stability of the protein .
TSG101 is a key component of the endosomal sorting complex required for transport (ESCRT) machinery, which is crucial for:
Role in Cancer
TSG101’s role in cancer is complex and context-dependent:
- Tumor Suppression: Initially, TSG101 was identified as a tumor suppressor gene. Its inactivation in mouse fibroblasts led to the formation of metastatic tumors .
- Tumor Enhancement: In some epithelial tumor cells, TSG101 functions as a tumor-enhancing gene. For example, TSG101 depletion can either promote or reduce cell invasion depending on the cell type .
Mechanisms of Action
TSG101 modulates cell invasion through the regulation of matrix metalloproteinase (MMP)-9 mRNA expression. This bidirectional modulation is cell-type specific:
Involvement in Viral Pathogenesis
Research and Therapeutic Potential
Given its diverse roles, TSG101 is a subject of extensive research. Understanding its mechanisms can provide insights into:
- Cancer therapy: Targeting TSG101 pathways could offer new therapeutic strategies.
- Viral infections: Inhibiting TSG101 interactions with viral proteins may help in developing antiviral treatments .
In conclusion, TSG101 is a multifunctional protein with significant implications in cancer biology and viral pathogenesis. Its dual role in tumor suppression and enhancement makes it a fascinating target for further research and potential therapeutic interventions.
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