TSC22D3 Human
TSC22 Domain Family, Member 3 Human Recombinant
Cat. No.
BT26832
Source
Escherichia Coli.
Synonyms
TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP, hDIP, Delta sleep-inducing peptide immunoreactor, Glucocorticoid-induced leucine zipper protein, GILZ, TSC-22-like protein, TSC-22-related protein, TSC-22R, TSC22D3, DSIPI, DIP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TSC22D3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids (1-134 a.a) and having a molecular mass of 17.2kDa.
TSC22D3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TSC22D3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
TSC22 domain family protein 3 (TSC22D3) plays a crucial role in protecting T-cells from apoptosis caused by IL2 deprivation. It achieves this by inhibiting the transcriptional activity of FOXO3A, which in turn suppresses the production of the pro-apoptotic factor BCL2L11. Furthermore, TSC22D3 is involved in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10 in macrophages. It also hinders the nuclear translocation of NFKB1 induced by anti-CD3 in T-cells. In laboratory settings, TSC22D3 has been observed to limit the DNA-binding activities of both AP1 and NFKB1.
Description
Recombinant human TSC22D3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 157 amino acids (specifically, amino acids 1 to 134). It possesses a molecular weight of 17.2 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of TSC22D3, and proprietary chromatographic techniques are employed.
Physical Appearance
The solution is sterile, filtered, and colorless.
Formulation
The TSC22D3 protein solution has a concentration of 1 mg/ml and is prepared in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, 10% glycerol, and 1mM DTT.
Stability
For optimal storage, keep the vial at 4°C if it will be used within 2-4 weeks. For prolonged storage, it is recommended to freeze the solution at -20°C. To ensure stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is advisable to minimize the number of freeze-thaw cycles.
Purity
SDS-PAGE analysis has determined the purity to be greater than 85.0%.
Synonyms
TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP, hDIP, Delta sleep-inducing peptide immunoreactor, Glucocorticoid-induced leucine zipper protein, GILZ, TSC-22-like protein, TSC-22-related protein, TSC-22R, TSC22D3, DSIPI, DIP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNTEMYQ TPMEVAVYQL HNFSISFFSS LLGGDVVSVK LDNSASGASV VAIDNKIEQA MDLVKNHLMY AVREEVEILK EQIRELVEKN SQLERENTLL KTLASPEQLE KFQSCLSPEE PAPESPQVPE APGGSAV.
Product Science Overview
Introduction
TSC22 Domain Family, Member 3 (TSC22D3), also known as Glucocorticoid-Induced Leucine Zipper (GILZ), is a protein encoded by the TSC22D3 gene in humans. This protein plays a crucial role in various biological processes, particularly in the regulation of inflammation and immune responses.
Gene and Protein Structure
Expression and Regulation
TSC22D3 expression is induced by glucocorticoids and interleukin-10 (IL-10). Glucocorticoids are steroid hormones that play a significant role in the regulation of metabolism, immune response, and stress. IL-10 is an anti-inflammatory cytokine that helps regulate immune responses. The expression of TSC22D3 is ubiquitous, meaning it is found in various tissues, including the thymus, spleen, lung, liver, kidney, heart, and skeletal muscle .
Function
TSC22D3 has several important functions:
- Anti-inflammatory and Immunosuppressive Effects: TSC22D3 inhibits the activity of pro-inflammatory molecules, such as nuclear factor κB (NF-κB), and suppresses the production of pro-inflammatory cytokines. This makes it a key player in the anti-inflammatory and immunosuppressive effects of glucocorticoids .
- Regulation of Apoptosis: TSC22D3 protects T-cells from apoptosis (programmed cell death) induced by IL-2 deprivation. It achieves this by inhibiting the transcriptional activity of FOXO3A, a transcription factor that promotes the expression of pro-apoptotic genes .
- Myogenic Differentiation: TSC22D3 inhibits myogenic differentiation by binding to and regulating the transcriptional activity of MYOD1 and HDAC1, leading to reduced expression of myogenic genes .
Clinical Significance
TSC22D3 is associated with various diseases and conditions:
- Hematologic Cancer: Altered expression of TSC22D3 has been linked to certain types of hematologic cancers .
- Epilepsy: There is evidence suggesting a potential role of TSC22D3 in epilepsy .
- Immune-Related Disorders: Due to its role in immune regulation, TSC22D3 is being studied for its potential involvement in immune-related disorders .
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