TRPT1 Human

The TRPT1 gene is a protein-coding gene that encodes the tRNA 2’-phosphotransferase 1 enzyme. The gene is located on chromosome 11 in humans and is identified by several aliases, including MGC11134 and EC 2.7.1.160 . The enzyme itself is predicted to have transferase activity, specifically transferring phosphorus-containing groups .

Recombinant human TRPT1 protein is produced by expressing the TRPT1 gene in a host organism, typically Escherichia coli (E. coli). The recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification. The amino acid sequence of the recombinant human TRPT1 includes the first 253 amino acids of the native protein .

The recombinant protein is purified using conventional chromatography techniques and is typically stored in a buffer containing Tris-HCl, glycerol, NaCl, and DTT to maintain stability .

TRPT1 catalyzes the last step of tRNA splicing, which is essential for the proper maturation of tRNA molecules. This process involves the transfer of the 2’-phosphate from the ligated tRNA to NAD, forming ADP-ribose 1’‘-2’’ cyclic phosphate . This step is critical for the proper functioning of tRNA, which in turn is essential for protein synthesis in cells.

Recombinant human TRPT1 is used in various research applications to study tRNA splicing and its role in cellular processes. It is also used to investigate the regulation of protein kinase activity, as TRPT1 is predicted to act upstream of or within this regulatory pathway .