Troponin-C2 Human

Troponin-C2 Human Recombinant

Cat. No.

BT18256

Source

Escherichia Coli.

Synonyms

Troponin C, skeletal muscle, TNNC2.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

Troponin-C2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain of 160 amino acids having a molecular mass of 18.1kDa. The Recombinant Human Troponin-C2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Troponin-C2 (Troponin C, skeletal muscle) is the central regulatory protein of striated muscle contraction and modulates the calcium ion activation properties of muscle fibers. Troponin-C2 is a complex of three subunits, Troponin I (Tn-I), Troponin T (Tn-T), and Troponin C (Tn-C). The Tn-I subunit inhibits actomyosin ATPase. The Tn-T subunit binds tropomyosin and Tn-C. The Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. Mutations in the genes encoding any of these subunits have been linked with skeletal muscle disease.

Description

Recombinant human Troponin-C2 produced in E. coli is a single, non-glycosylated polypeptide chain of 160 amino acids with a molecular mass of 18.1 kDa. The recombinant human Troponin-C2 protein is purified by proprietary chromatographic techniques.

Physical Appearance

Clear, colorless, and sterile-filtered solution.

Formulation

The protein (1 mg/mL) is supplied in 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 100 mM NaCl, and 10% glycerol.

Stability

For short-term storage, store at 4°C for up to 4 weeks. For long-term storage, store at -20°C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Synonyms

Troponin C, skeletal muscle, TNNC2.

Source

Escherichia Coli.

Amino Acid Sequence

MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT PTKEELDAII EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF RIFDRNADGY IDPEELAEIF RASGEHVTDE EIESLMKDGD KNNDGRIDFD EFLKMMEGVQ.

Product Science Overview

Structure and Function

Troponin-C2 is part of the troponin complex, which consists of three subunits:

Troponin I (Tn-I): Inhibits actomyosin ATPase.
Troponin T (Tn-T): Binds tropomyosin and Troponin C.
Troponin C (Tn-C): Binds calcium ions and overcomes the inhibitory action of the troponin complex on actin filaments .

The binding of calcium to Troponin-C2 induces a conformational change that allows the interaction between actin and myosin, leading to muscle contraction. This process is essential for the proper functioning of skeletal muscles .

Recombinant Human Troponin-C2

Recombinant human Troponin-C2 is produced using Escherichia coli (E. coli) as the expression system. The recombinant protein corresponds to the amino acids 1-160 of the human TNNC2 gene . It is purified using conventional chromatography techniques to achieve a purity of over 95%.

The recombinant protein is typically stored in a buffer containing Tris-HCl, DTT, NaCl, and glycerol to maintain its stability and activity .

Applications and Research

Recombinant human Troponin-C2 is widely used in research to study muscle physiology, muscle diseases, and the mechanisms of muscle contraction. It is also used in the development of diagnostic assays and therapeutic interventions for muscle-related disorders .

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Troponin-C2 Human

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