Tpc1808 Rat

Tropic 1808 is a single, non-glycosylated polypeptide chain consisting of 285 amino acids, with a molecular mass of approximately 29.1 kDa . The protein is produced in Escherichia coli (E. coli) and is typically fused to an N-terminal His-Tag to facilitate purification . The protein is purified using high-performance liquid chromatography (HPLC) and validated for bioactivity through various assays .

Tropic 1808 is considered a candidate chemotropic factor, which means it can influence the direction of nerve growth. It is induced by nerve injury and has been shown to promote the expression of neurofilament heavy chain (NF-H) in a time-dependent manner . This activity is similar to that of nerve growth factor (NGF), a well-known protein involved in the growth, maintenance, and survival of neurons .

Due to its role in promoting nerve growth, Tropic 1808 is used in various research applications, particularly in studies focused on nerve injury and regeneration. It has been shown to up-regulate the expression of NF-H in PC12 cells, a commonly used cell line in neurobiological research . This makes it a valuable tool for understanding the mechanisms of nerve growth and developing potential therapeutic strategies for nerve injuries.

Tropic 1808 is typically lyophilized (freeze-dried) and should be stored at temperatures below -18°C to maintain its stability . It is important to avoid freeze-thaw cycles to prevent degradation of the protein. The lyophilized protein is reconstituted in a buffer solution, usually phosphate-buffered saline (PBS) at pH 7.4, before use .