TRIP10 Human

Thyroid Hormone Receptor Interactor 10 Human Recombinant
Cat. No.
BT26722
Source
Escherichia Coli.
Synonyms
Cdc42-interacting protein 4, Protein Felic, Salt tolerant protein, hSTP, Thyroid receptor-interacting protein 10, TR-interacting protein 10, TRIP-10,TRIP10, CIP4, STOT, STP, HSTP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TRIP10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 309 amino acids (260-545) and having a molecular mass of 34.6 kDa.
TRIP10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Thyroid Hormone Receptor Interactor 10 (TRIP10), a member of the F-BAR protein family, is widely distributed in tissues such as the kidney, brain, liver, lung, heart, and pancreas. This family is characterized by an N-terminal alpha-helical region that exhibits hydrophobic properties and shares similarities with the Bin Amphiphysin Rvs (BAR) protein family. TRIP10 plays a crucial role in coordinating membrane tubulation with actin cytoskeletal reorganization during the process of endocytosis.
Description
Recombinant human TRIP10, expressed in E. coli, is a monomeric, non-glycosylated polypeptide chain encompassing amino acids 260 to 545, resulting in a molecular weight of 34.6 kDa. This protein construct consists of TRIP10 fused to a 23-amino acid His-tag at its N-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile filtered, colorless solution.
Formulation
The TRIP10 solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. To ensure long-term stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0% as assessed by SDS-PAGE analysis.
Synonyms
Cdc42-interacting protein 4, Protein Felic, Salt tolerant protein, hSTP, Thyroid receptor-interacting protein 10, TR-interacting protein 10, TRIP-10,TRIP10, CIP4, STOT, STP, HSTP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSDPKNDSH VLIELHKSGF ARPGDVEFED FSQPMNRAPS DSSLGTPSDG RPELRGPGRS RTKRWPFGKK NKTVVTEDFS HLPPEQQRKR LQQQLEERSR ELQKEVDQRE ALKKMKDVYE KTPQMGDPAS LEPQIAETLS NIERLKLEVQ KYEAWLAEAE SRVLSNRGDS LSRHARPPDP PASAPPDSSS NSASQDTKES SEEPPSEESQ DTPIYTEFDE DFEEEPTSPI GHCVAIYHFE GSSEGTISMA EGEDLSLMEE DKGDGWTRVR RKEGGEGYVP TSYLRVTLN.

Product Science Overview

Gene and Protein Information

The TRIP10 gene is located on chromosome 19 and is a protein-coding gene . The protein encoded by this gene is involved in the translocation of GLUT4 to the plasma membrane in response to insulin signaling . It also coordinates membrane tubulation with the reorganization of the actin cytoskeleton during endocytosis .

Function and Mechanism

TRIP10 binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine, promoting membrane invagination and the formation of tubules . It also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP, which in turn activates the Arp2/3 complex . This actin polymerization may promote the fission of membrane tubules to form endocytic vesicles . Additionally, TRIP10 is required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells .

Clinical Significance

TRIP10 has been identified as a biomarker for Huntington’s disease . It is also associated with Wiskott-Aldrich Syndrome, a rare genetic disorder that affects the immune system and blood clotting . The protein’s involvement in various signaling pathways, such as those mediated by Rho GTPases and vesicle-mediated transport, underscores its importance in cellular function and disease .

Expression and Localization

TRIP10 is expressed in various tissues, including the hippocampal formation, amygdala, basal ganglia, midbrain, spinal cord, cerebral cortex, cerebellum, hypothalamus, and choroid plexus . It is predominantly localized to the cytoplasm and vesicles, with additional localization to the nucleoplasm .

Research and Applications

The recombinant form of TRIP10 is used in research to study its role in cellular processes and its involvement in diseases. Understanding the function and mechanism of TRIP10 can provide insights into potential therapeutic targets for diseases associated with this protein.

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