TRIAP1 Human

TP53 Regulated Inhibitor Of Apoptosis 1 Human Recombinant

Cat. No.

BT26649

Source

Escherichia Coli.

Synonyms

TP53 Regulated Inhibitor of Apoptosis 1, P53-Inducible Cell-Survival Factor, Mitochondrial Distribution And Morphology 35 Homolog, Protein 15E1.1, MDM35, WF-1, p53CSV, HSPC132.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TRIAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 99 amino acids (1-76 a.a) and having a molecular mass of 11.2kDa.

TRIAP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

TRIAP1, a protein with a p53-binding site in its second exon, plays a role in apoptosis regulation. Its expression is reduced by small interfering RNA, leading to enhanced apoptosis. Conversely, TRIAP1 overexpression protects cells from apoptosis induced by DNA damage. Interestingly, TRIAP1 is highly induced in response to low levels of genotoxic stress but not severe DNA damage. It regulates apoptotic pathways through interaction with Hsp70, which inhibits the activity of apoptosis protease activating factor-1.

Description

Recombinant human TRIAP1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 99 amino acids (residues 1-76). It has a molecular mass of 11.2 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, sterile-filtered solution.

Formulation

The TRIAP1 protein solution (1 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 1 mM DTT, and 20% glycerol.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.

Purity

Purity greater than 90.0% as determined by SDS-PAGE.

Synonyms

TP53 Regulated Inhibitor of Apoptosis 1, P53-Inducible Cell-Survival Factor, Mitochondrial Distribution And Morphology 35 Homolog, Protein 15E1.1, MDM35, WF-1, p53CSV, HSPC132.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMNSVGEA CTDMKREYDQ CFNRWFAEKF LKGDSSGDPC TDLFKRYQQC VQKAIKEKEI PIEGLEFMGH GKEKPENSS

Product Science Overview

Introduction

TP53 Regulated Inhibitor Of Apoptosis 1 (TRIAP1), also known as p53CSV or WF-1, is a protein encoded by the TRIAP1 gene in humans. This protein plays a crucial role in the regulation of apoptosis, a process of programmed cell death that is essential for maintaining cellular homeostasis and preventing cancer development.

Gene and Protein Structure

The TRIAP1 gene is located on chromosome 12 and encodes a small, evolutionarily conserved protein consisting of 76 amino acids. The protein is primarily found in the mitochondrial intermembrane space and the nucleoplasm, where it forms part of a protein-containing complex.

Function and Mechanism

TRIAP1 is involved in several critical cellular processes, including:

DNA Damage Response: It contributes to the DNA damage response by interacting with the p53 protein, a well-known tumor suppressor.
Apoptosis Regulation: TRIAP1 inhibits the activation of caspase-9, a key enzyme in the apoptotic pathway, thereby preventing the induction of apoptosis.
Phospholipid Transport: The TRIAP1:PRELID1 and TRIAP1:PRELID3A complexes mediate the transfer of phosphatidic acid (PA) between liposomes, ensuring the accumulation of cardiolipin (CL) in mitochondrial membranes.

Role in Cancer

TRIAP1 has been shown to be upregulated in various types of cancers, including breast cancer. Its overexpression is associated with increased drug resistance, making it a potential target for cancer therapy. By binding to HSP70 in the cytoplasm, TRIAP1 blocks the formation of the apoptosome and caspase-9 activation, thereby promoting cell survival and drug resistance.

Research and Therapeutic Potential

Research has demonstrated that downregulation of TRIAP1 impairs cell growth in the presence of chemotherapeutic agents like doxorubicin, highlighting its potential as a therapeutic target. Strategies to inhibit TRIAP1 activity could enhance the effectiveness of cancer treatments and overcome drug resistance.

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TRIAP1 Human

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