Sf9 insect cells.
Non-specific lipid-transfer protein, ltp142.
Sterile Filtered clear solution.
Greater than 80.0% as determined by SDS-PAGE.
Recombinant Non-Specific Lipid-Transfer Protein Tri a 14 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 13kDa.
Tri a 14.0101 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Non-specific lipid-transfer protein, ltp142.
Sf9 insect cells.
Non-Specific Lipid-Transfer Protein (nsLTP) Tri a 14 is a significant allergen found in wheat (Triticum aestivum). It is known for its role in various allergic reactions, including food allergies, baker’s asthma, and wheat-dependent exercise-induced anaphylaxis (WDEIA). The recombinant form of this protein, referred to as Tri a 14 Recombinant, has been developed to study its properties and potential applications in allergy diagnostics and research.
Tri a 14 is a 9-kDa protein that belongs to the nsLTP family. These proteins are characterized by their ability to bind and transfer lipids between membranes. Tri a 14 is found in the grains of wheat and is present in higher concentrations in the bran compared to the flour . The protein is stable to heat and digestion, making it a persistent allergen even in processed foods .
Tri a 14 is a major allergen associated with baker’s asthma, a common occupational allergy among individuals exposed to wheat flour . Sensitization to Tri a 14 can occur through ingestion or inhalation of wheat proteins. The protein has been shown to elicit strong IgE-mediated immune responses in sensitized individuals . Studies have demonstrated that both natural and recombinant forms of Tri a 14 have similar immunological properties, making the recombinant form a valuable tool for allergy diagnostics .
The recombinant form of Tri a 14 is produced using the yeast Pichia pastoris. The cDNA encoding Tri a 14 is isolated and inserted into the yeast expression system. The recombinant protein is then purified using chromatographic methods . This process ensures that the recombinant protein retains its structural and immunological properties, making it suitable for use in diagnostic assays.
Recombinant Tri a 14 has been evaluated as a diagnostic tool for baker’s asthma and other wheat-related allergies. It has been shown to have high heat and proteolytic resistance, similar to its natural counterpart . This stability makes it a reliable component for diagnostic tests. The use of recombinant Tri a 14 in component-resolved diagnostics allows for more accurate identification of specific allergens responsible for allergic reactions .