TRDMT1 Human

tRNA Aspartic Acid Methyltransferase 1 Human Recombinant
Cat. No.
BT15123
Source
Escherichia Coli.
Synonyms
tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, M.HsaIIP, PuMet, TRDMT1, DNMT2, DMNT2, RNMT1, MHSAIIP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TRDMT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 415 amino acids (1-391) and having a molecular mass of 47.2kDa.
TRDMT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
tRNA aspartic acid methyltransferase 1 (TRDMT1), a member of the C5-methyltransferase family, specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). This enzyme is responsible for methylating aspartic acid transfer RNA, particularly at the cytosine-38 residue in the anticodon loop. Additionally, TRDMT1 exhibits residual DNA-(cytosine-C5) methyltransferase activity. Ubiquitously expressed, TRDMT1 shows higher expression in the testis, ovary, and thymus, with lower levels detected in the spleen, prostate, colon, small intestine, and peripheral blood leukocytes. While similar in sequence and structure to DNA cytosine methyltransferases, the TRDMT1 gene is distinct and highly conserved in its function across different species.
Description
Recombinant Human TRDMT1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 415 amino acids (1-391). It has a molecular weight of 47.2 kDa. The protein includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The TRDMT1 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the product is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, M.HsaIIP, PuMet, TRDMT1, DNMT2, DMNT2, RNMT1, MHSAIIP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE.

Product Science Overview

Gene and Protein Information

The TRDMT1 gene is located on chromosome 10 in humans and is highly conserved across different species. The protein encoded by this gene is similar in sequence and structure to DNA cytosine methyltransferases, but it has a distinct function. Unlike typical DNA methyltransferases, TRDMT1 primarily targets RNA rather than DNA .

Function and Mechanism

TRDMT1 is responsible for the methylation of cytosine residues in tRNA, which is essential for the proper functioning of tRNA molecules. This methylation process is crucial for the stability and proper folding of tRNA, which in turn affects protein synthesis. The enzyme has higher activity on tRNA (Asp) that is modified with queuosine at position 34 .

Clinical Significance

Mutations or dysregulation of the TRDMT1 gene have been associated with various diseases, including diffuse alopecia areata and trigonitis. The enzyme’s role in RNA methylation also implicates it in the recognition of DNA damage, DNA recombination, and mutation repair through both DNA and RNA methylation pathways .

Research and Applications

Human recombinant TRDMT1 is widely used in research to study RNA methylation and its effects on cellular processes. Understanding the function and regulation of this enzyme can provide insights into the mechanisms of gene expression and the development of certain diseases. Additionally, TRDMT1 is a potential target for therapeutic interventions aimed at modulating RNA methylation in disease contexts .

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