TPRKB Human
TP53RK Binding Protein Human Recombinant
Cat. No.
BT26411
Source
E.coli.
Synonyms
TP53RK binding protein, PRPK-binding protein, PRPK (p53-related protein kinase)-binding protein, CGI-121.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TPRKB Human Recombinant produced E. coli is a single polypeptide chain containing 199 amino acids (1-175) and having a molecular mass of 22.2kDa.
TPRKB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
TPRKB is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
TPRKB, a member of the CGI121/TPRKB family, is located in both the nucleus and cytoplasm of cells. It is found throughout the body and is known to work with TP53RK/PRPK.
Description
Recombinant human TPRKB, produced in E. coli bacteria, is a single chain of 199 amino acids (specifically amino acids 1 through 175) with a molecular weight of 22.2kDa. It has a 24 amino acid His-tag attached to its N-terminus and is purified using specialized chromatographic methods.
Physical Appearance
A clear, sterile solution.
Formulation
The TPRKB solution has a concentration of 0.5mg/ml and is stored in a buffer containing 20mM Tris-HCl (pH 8.0), 150mM NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing is not recommended.
Purity
Purity is greater than 95% as measured by SDS-PAGE.
Synonyms
TP53RK binding protein, PRPK-binding protein, PRPK (p53-related protein kinase)-binding protein, CGI-121.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMQLTHQ LDLFPECRVT LLLFKDVKNA GDLRRKAMEG TIDGSLINPT VIVDPFQILV AANKAVHLYK LGKMKTRTLS TEIIFNLSPN NNISEALKKF GISANDTSIL IVYIEEGEKQ INQEYLISQV EGHQVSLKNL PEIMNITEVK KIYKLSSQEE SIGTLLDAII CRMSTKDVL
Product Science Overview
Introduction
Gene and Protein Structure
Function and Mechanism
TP53RK is an upstream kinase that phosphorylates the serine residue Ser15 on the p53 protein, thereby mediating its activity . This phosphorylation event is crucial for the activation of p53 in response to DNA damage and other genotoxic stressors . TP53RK also exhibits ATPase activity within the context of the EKC/KEOPS complex, which is involved in the biosynthesis of N-6-threonylcarbamoyladenosine (t6A) in the cytosol .
Biological Significance
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