TPMT Antibody

Thiopurine S-methyltransferase, Mouse Anti Human
Cat. No.
BT26583
Source
Synonyms
TPMT, Thiopurine S-methyltransferase, EC 2.1.1.67, Thiopurine methyltransferase.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme crucial for metabolizing thiopurine drugs. It utilizes S-adenosyl-L-methionine as the methyl donor, producing S-adenosyl-L-homocysteine as a byproduct. TPMT activity displays autosomal codominant genetic polymorphism. Individuals with inherited TPMT deficiency are highly susceptible to potentially lethal hematopoietic toxicity.
Formulation
The antibody is provided at a concentration of 1 mg/ml in a buffer solution of phosphate-buffered saline (PBS) at pH 7.4, with 0.1% sodium azide added as a preservative.
Storage Procedures
For short-term storage of up to one month, keep the antibody at 4°C. For prolonged storage, store at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The TPMT antibody has undergone rigorous testing through ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. A recommended starting dilution for Western blot analysis is 1:1,000.
Synonyms
TPMT, Thiopurine S-methyltransferase, EC 2.1.1.67, Thiopurine methyltransferase.
Purification Method
TPMT antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT2E7AT.
Immunogen
Anti-human TPMT mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human TPMT amino acids 1-245 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Background of Mouse Anti-Human Antibodies

Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are widely used in various applications, including detection, sorting, and purification of human proteins .

Applications and Benefits

Mouse anti-human antibodies are versatile tools in biomedical research and diagnostics. They can be conjugated with various labels, such as enzymes (HRP, AP), fluorophores (FITC, PE), or biotin, to facilitate detection and quantification of target proteins . These secondary antibodies enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody .

Human Anti-Mouse Antibody (HAMA) Response

One potential issue with using mouse anti-human antibodies is the development of a human anti-mouse antibody (HAMA) response . This immune response can range from mild allergic reactions to severe complications, such as kidney failure . Therefore, it is essential to monitor patients for HAMA responses when using mouse-derived antibodies in clinical settings .

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Related Products

TPMT Human
Thiopurine S-methyltransferase Human Recombinant
Cat. No.
BT15283
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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