TOMM20 Antibody
Mmitochondrial import receptor subunit TOM20 homolog, TOMM20, MAS20, MOM19, KIAA0016, Outer mitochondrial membrane receptor Tom20, Mitochondrial 20 kDa outer membrane protein.
Description
Product Specs
Mmitochondrial import receptor subunit TOM20 homolog, TOMM20, MAS20, MOM19, KIAA0016, Outer mitochondrial membrane receptor Tom20, Mitochondrial 20 kDa outer membrane protein.
TOMM20 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT1B2AT.
Anti-human TOMM20 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TOMM20 protein 25-145 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
The translocase of the outer mitochondrial membrane 20 (TOMM20) is a crucial protein involved in the import of mitochondrial preproteins. It is part of the TOM complex, which facilitates the translocation of proteins across the outer mitochondrial membrane. The TOM complex is essential for mitochondrial function and biogenesis, as it ensures that proteins synthesized in the cytosol are correctly imported into the mitochondria.
TOMM20 is a receptor protein located in the outer mitochondrial membrane. It plays a pivotal role in recognizing and binding to the presequence of mitochondrial precursor proteins. The TOM complex consists of several components, including TOM20, TOM22, TOM40, and TOM70. TOM20, along with TOM22, acts as a receptor for the presequence of mitochondrial proteins, guiding them to the TOM40 translocation pore .
The import of mitochondrial proteins is a multi-step process. Initially, TOM20 recognizes and binds to the presequence of the precursor protein. This interaction is followed by the transfer of the presequence to TOM22, which further guides the protein to the TOM40 channel. The protein is then translocated across the outer membrane and handed over to the translocase of the inner membrane (TIM) complex for further import into the mitochondrial matrix .
The mouse anti-human TOMM20 antibody is a monoclonal antibody produced in mice that specifically targets the human TOMM20 protein. This antibody is widely used in research to study mitochondrial function, protein import mechanisms, and mitochondrial diseases. It is also utilized in various applications, including Western blotting, immunoprecipitation, and immunofluorescence .
- Western Blotting: The mouse anti-human TOMM20 antibody is used to detect TOMM20 protein levels in cell and tissue samples. This helps in understanding the expression and regulation of TOMM20 in different conditions.
- Immunoprecipitation: This technique allows the isolation and study of TOMM20 protein complexes, providing insights into its interactions with other mitochondrial proteins.
- Immunofluorescence: The antibody is used to visualize the localization of TOMM20 within cells, aiding in the study of mitochondrial distribution and dynamics .
TOMM20 is essential for mitochondrial biogenesis and function. Defects in the TOM complex can lead to impaired protein import, resulting in mitochondrial dysfunction and associated diseases. Studying TOMM20 and its interactions with other mitochondrial proteins is crucial for understanding the molecular mechanisms underlying mitochondrial diseases and developing potential therapeutic strategies .
