TNC Rabbit

Troponin-C is a single, glycosylated polypeptide chain with a molecular mass of approximately 18 kDa . It is composed of four EF-hand motifs, which are helix-loop-helix structures capable of binding calcium ions. The binding of calcium to Troponin-C induces a conformational change that allows the interaction of the troponin complex with tropomyosin and actin, leading to muscle contraction .

Troponin-C from rabbit skeletal muscle is typically isolated using a combination of ion-exchange and affinity chromatography steps. The protein is provided as a sterile filtered, colorless solution containing 150 mM sodium chloride, 10 mM sodium phosphate, and 0.05% sodium azide at pH 7.2 . The purity of the isolated protein is greater than 95%, as determined by SDS-PAGE .

Rabbit skeletal muscle Troponin-C is widely used in biochemical and physiological studies to understand muscle contraction mechanisms. It is also used in the development of diagnostic assays and in the production of antibodies for research purposes .

For optimal stability, Troponin-C should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .