TNIP1 Human

TNFAIP3 Interacting Protein 1 Human Recombinant
Cat. No.
BT25637
Source
Escherichia Coli.
Synonyms
TNFAIP3-interacting protein 1, HIV-1 Nef-interacting protein, Nef-associated factor 1, Naf1, Nip40-1, Virion-associated nuclear shuttling protein, VAN, hVAN, TNIP1, KIAA0113, NAF1, ABIN-1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 75.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TNIP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 458 amino acids (94-530 a.a.) and having a molecular mass of 51.8kDa. The TNIP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
TNFAIP3-interacting protein 1 (TNIP1) interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with a RIP- or TRAF2-mediated transactivation signal. Additionally, TNIP1 interacts with the HIV-1 matrix protein, becoming incorporated into virions. Its overexpression can hinder viral replication. TNIP1 can regulate matrix nuclear localization, impacting both the nuclear import of the Preintegration complex (PIC) and the export of GAG polyprotein and viral genomic RNA during virion production.
Description
Recombinant human TNIP1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein, fused with a 20 amino acid His tag at its N-terminus, encompasses amino acids 94-530 and has a molecular weight of 51.8 kDa. Purification of TNIP1 is achieved using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
TNIP1 is supplied in a solution at a concentration of 0.25 mg/ml. The solution is buffered with 20mM Tris-HCl at pH 7.5 and contains 20% glycerol, 5mM DTT, and 200mM NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein like HSA or BSA (0.1%). Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 75.0% using SDS-PAGE analysis.
Synonyms
TNFAIP3-interacting protein 1, HIV-1 Nef-interacting protein, Nef-associated factor 1, Naf1, Nip40-1, Virion-associated nuclear shuttling protein, VAN, hVAN, TNIP1, KIAA0113, NAF1, ABIN-1.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MSNVTASPTA PACPSDKPAP VQKPPSSGTS SEFEVVTPEE QNSPESSSHA NAMALGPLPR EDGNLMLHLQ RLETTLSVCA EEPDHGQLFT HLGRMALEFN RLASKVHKNE QRTSILQTLC EQLRKENEAL KAKLDKGLEQ RDQAAERLRE ENLELKKLLM SNGNKEGASG RPGSPKMEGT GKKAVAGQQQ ASVTAGKVPE VVALGAAEKK VKMLEQQRSE LLEVNKQWDQ HFRSMKQQYE QKITELRQKL ADLQKQVTDL EAEREQKQRD FDRKLLLAKS KIEMEETDKE QLTAEAKELR QKVKYLQDQL SPLTRQREYQ EKEIQRLNKA LEEALSIQTP PSSPPTAFGS PEGAGALLRK QELVTQNELL KQQVKIFEED FQRERSDRER MNEEKEELKK QVEKLQAQVT LSNAQLKAFK DEEKAREALR QQKRKAKA.

Product Science Overview

Gene and Protein Information

The TNIP1 gene is located on chromosome 5 and encodes a protein that interacts with the A20 protein (TNFAIP3). This interaction is significant for the regulation of nuclear factor kappa-B (NF-κB) activation, which is a key pathway in inflammatory and immune responses .

Function and Mechanism

TNIP1 inhibits NF-κB activation and TNF-induced NF-κB-dependent gene expression by regulating the deubiquitination of IKBKG through its interaction with TAX1BP1 and A20/TNFAIP3 . This regulation is essential for controlling inflammation and preventing autoimmune diseases. TNIP1 also plays a role in the EGF-induced ERK1/ERK2 signaling pathway, which is involved in cell growth and differentiation .

Structural Characteristics

TNIP1 is an intrinsically disordered protein, meaning it lacks a fixed or ordered three-dimensional structure under physiological conditions. This structural flexibility is contributed by its AHD1-UBAN domain, which allows TNIP1 to interact with various cellular, viral, and bacterial proteins . The AHD1-UBAN domain exists primarily as a pre-molten globule with limited secondary structure in solution, which may impact its function and interaction with multiple partners .

Clinical Significance

Mutations in the TNIP1 gene have been associated with several autoimmune diseases, including psoriatic arthritis, rheumatoid arthritis, and systemic lupus erythematosus . The protein’s role in regulating immune responses makes it a potential target for therapeutic interventions in these conditions.

Research and Applications

Research on TNIP1 has shown its involvement in various cellular processes and its potential as a therapeutic target. For instance, TNIP1’s interaction with the HIV-1 matrix protein suggests a role in viral replication and potential applications in antiviral therapies . Additionally, its regulation of leukocyte integrin activation during inflammation highlights its importance in immune response modulation .

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