TNFSF7 Human
Description
Product Specs
Product Science Overview
CD70 is a cell surface antigen involved in lymphocyte maturation and proliferation . The protein consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain of human CD70 spans amino acids 39 to 193 . Recombinant human CD70 is often produced in Chinese Hamster Ovary (CHO) cells or HEK293 cells and purified by affinity chromatography .
The primary function of CD70 is to bind to its receptor, CD27, which is expressed on T cells . The CD70-CD27 interaction plays a crucial role in T cell activation, differentiation, and survival . This interaction also promotes the generation of memory T cells and plasma cells, contributing to long-term immune responses .
CD70 expression is tightly regulated to prevent excessive immune activation, which could lead to immunodeficiency or autoimmunity . In normal tissues, CD70 expression is transient and restricted to activated lymphocytes and dendritic cells . However, aberrant expression of CD70 has been observed in various malignancies, making it an attractive target for cancer immunotherapy .
Recombinant human CD70 is used in various research applications, including:
- Screening of high-affinity anti-human CD70 monoclonal antibodies by enzyme-linked immunosorbent assay (ELISA) .
- Flow cytometry to validate protein expression and potency using cell lines such as Jurkat-Lucia™ hCD27 cells .
- Cancer immunotherapy research, where CD70 is targeted to develop antibody-based therapeutics .
Recombinant human CD70 is typically lyophilized and can be reconstituted in sterile distilled water . It is recommended to store the lyophilized protein at -20°C to -80°C for long-term stability . Once reconstituted, the protein solution is stable at -20°C for up to three months and at 2-8°C for up to one week .
