CD27 Human, HEK
HEK293 Cells.
Tumor Necrosis Factor Receptor Superfamily Member 7, T-Cell Activation Antigen CD27, CD27 Molecule, CD27 Antigen, T Cell Activation Antigen S152, CD27L Receptor, TNFRSF7, S152, Tp55, T14.
Sterile Filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
CD27 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (20-191 a.a) containing a total of 411 amino acids, having a molecular mass of 46.2kDa.
CD27 is fused to a 239 amino acid hIgG-His-Tag at C-terminus,and is purified by proprietary chromatographic techniques.
Product Specs
A member of the TNF-receptor superfamily, CD27 is essential for both the initiation and long-term maintenance of T cell immunity. This receptor binds to the ligand CD70 and plays a key role in regulating B-cell activation and the production of immunoglobulins. Signal transduction through the CD27 receptor leads to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 mediate this signaling process. CD27-binding protein (SIVA), a proapoptotic protein, can bind to the CD27 receptor and is thought to play a significant role in CD27-induced apoptosis.
Recombinant Human CD27, produced in HEK cells, is a single, glycosylated polypeptide chain. It encompasses amino acids 20-191 and includes a C-terminal fusion of a 239 amino acid hIgG-His-Tag, resulting in a total of 411 amino acids and a molecular mass of 46.2kDa. Purification is achieved using proprietary chromatographic techniques.
The product is a sterile filtered solution, colorless in appearance.
The CD27 solution is provided at a concentration of 0.5mg/ml. It is formulated in a solution containing 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. Long-term storage requires freezing at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Repeated freeze-thaw cycles should be avoided.
Purity is determined by SDS-PAGE analysis and is guaranteed to be greater than 95.0%.
The ED50, determined by a functional ELISA utilizing Human CD27 Ligand/TNFSF7 (CAT# cyt-953), is ≤ 0.8 ug/ml. This assay measures the binding ability of the recombinant protein.
Tumor Necrosis Factor Receptor Superfamily Member 7, T-Cell Activation Antigen CD27, CD27 Molecule, CD27 Antigen, T Cell Activation Antigen S152, CD27L Receptor, TNFRSF7, S152, Tp55, T14.
HEK293 Cells.
ATPAPKSCPE RHYWAQGKLC CQMCEPGTFL VKDCDQHRKA AQCDPCIPGV SFSPDHHTRP HCESCRHCNS GLLVRNCTIT ANAECACRNG WQCRDKECTE CDPLPNPSLT ARSSQALSPH PQPTHLPYVS EMLEARTAGH MQTLADFRQL PARTLSTHWP PQRSLCSSDF IRLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H
Product Science Overview
CD27, also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7), is a protein encoded by the CD27 gene. This receptor is crucial for the generation and long-term maintenance of T cell immunity. It binds to its ligand, CD70, and plays a significant role in regulating B-cell activation and immunoglobulin synthesis .
Recombinant human CD27 protein is typically expressed in HEK 293 cells, a human embryonic kidney cell line. The protein is often produced as a fragment, ranging from amino acids 21 to 192, and can be expressed with various tags such as Fc or His tags for purification and detection purposes . The molecular weight of the monomeric form of CD27 is approximately 45.8 kDa, although it may run at a higher molecular weight in SDS-PAGE due to glycosylation .
CD27 is a receptor for CD70 (CD27L) and is involved in several critical immune processes:
- T Cell Activation and Survival: CD27 plays a role in the survival of activated T cells, which are essential for adaptive immunity.
- Apoptosis: It may also be involved in apoptosis through its association with the protein SIVA1 .
- B Cell Activation: CD27 is important for B-cell activation and the synthesis of immunoglobulins, which are crucial for the body’s defense against pathogens .
Recombinant CD27 protein is used in various research applications, including:
- Functional Studies: To study the binding interactions between CD27 and CD70, and to understand the signaling pathways involved in T cell and B cell activation.
- ELISA: As a standard in enzyme-linked immunosorbent assays (ELISA) to quantify the presence of CD27 or its ligands in biological samples .
- SDS-PAGE: For analyzing the purity and molecular weight of the protein .
