TNFRSF25 Human

TNF Ligand Receptor Superfamily Member 25 Recombinant Human
Cat. No.
BT28420
Source
Sf9, Baculovirus cells.
Synonyms
Tumor necrosis factor receptor superfamily member 25, TNFRSF25, TNF Ligand Receptor Superfamily Member 25, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR, Apo-3, Apoptosis-inducing receptor AIR, Protein WSL, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TNFRSF25 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 417 amino acids (25-199) and having a molecular mass of 46.1kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). TNFRSF25 is fused to a 242 amino acid IgG His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
TNF Ligand Receptor Superfamily Member 25 (TNFRSF25) is part of the TNF receptor family and binds to the TNF-like protein TL1A. It directly interacts with the adapter protein TRADD, playing a role in regulating lymphocyte balance. TNFRSF25 also participates in activating NF-kappa-B and can trigger apoptosis. Its signals are crucial for T helper cell 2 effector function in Th2-polarized CD4 cells and co-stimulate interleukin-13 production by glycosphingolipid-activated NKT cells.
Description
Recombinant Human TNFRSF25, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It consists of 417 amino acids (25-199) and has a molecular weight of 46.1kDa. Note that on SDS-PAGE, the apparent molecular size will be approximately 40-57kDa. The protein is fused to a 242 amino acid IgG His-Tag at its C-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The TNFRSF25 protein solution is provided at a concentration of 0.25mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C is recommended. To further enhance long-term stability, consider adding a carrier protein (0.1% HSA or BSA). It's important to avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Biological Activity

Biological activity is assessed through the protein's binding ability in a functional ELISA utilizing Human VEGI (CAT# cyt-589). The ED50 is determined to be less than or equal to 5ug/ml.

Synonyms
Tumor necrosis factor receptor superfamily member 25, TNFRSF25, TNF Ligand Receptor Superfamily Member 25, APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR, Apo-3, Apoptosis-inducing receptor AIR, Protein WSL, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPQGGTRSP RCDCAGDFHK KIGLFCCRGC PAGHYLKAPC TEPCGNSTCL VCPQDTFLAW ENHHNSECAR CQACDEQASQ VALENCSAVA DTRCGCKPGW FVECQVSQCV SSSPFYCQPC LDCGALHRHT RLLCSRRDTD CGTCLPGFYE HGDGCVSCPT STLGSCPERC AAVCGWRQLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.

Product Science Overview

Structure and Expression

TNFRSF25 is a glycoprotein that is expressed on the surface of various immune cells, including T cells, B cells, and natural killer (NK) cells. The receptor is characterized by the presence of cysteine-rich domains in its extracellular region, which are typical of the TNF receptor superfamily. The intracellular region of TNFRSF25 contains a death domain, which is crucial for the initiation of apoptotic signaling pathways .

Function

The primary function of TNFRSF25 is to mediate apoptosis and regulate immune responses. Upon binding to its ligand, TNFRSF25 can activate several downstream signaling pathways, including the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway and the mitogen-activated protein kinase (MAPK) pathway. These signaling pathways play essential roles in cell survival, proliferation, and differentiation .

TNFRSF25 is also involved in the regulation of T helper cell 2 (Th2) effector activity. It co-stimulates the production of interleukin-13 (IL-13) by glycosphingolipid-activated natural killer T (NKT) cells, which is vital for the immune response against certain pathogens .

Recombinant Human TNFRSF25

Recombinant human TNFRSF25 is a laboratory-produced version of the naturally occurring receptor. It is created using recombinant DNA technology, which involves inserting the gene encoding TNFRSF25 into a suitable expression system, such as bacteria or mammalian cells. The recombinant receptor is then purified and used for various research and therapeutic purposes.

Applications in Research and Medicine

Recombinant human TNFRSF25 is widely used in research to study its role in immune regulation and apoptosis. It is also used to investigate the signaling pathways activated by TNFRSF25 and to identify potential therapeutic targets for diseases involving dysregulated apoptosis and immune responses.

In medicine, recombinant human TNFRSF25 has potential therapeutic applications in the treatment of autoimmune diseases, cancer, and other conditions characterized by abnormal immune responses. By modulating the activity of TNFRSF25, it may be possible to restore normal immune function and induce apoptosis in cancer cells .

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