TNFRSF21 Human
Sf9, Baculovirus cells.
Tumor necrosis factor receptor superfamily member 21, BM-018, CD358, DR6, Death receptor 6.
Sterile filtered colorless solution.
Greater than 85.0% as determined by SDS-PAGE.
Description
TNFRSF21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 547 amino acids (42-349a.a.) and having a molecular mass of 60.4kDa. (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
TNFRSF21 is expressed with a 239 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
TNFRSF21, also known as Tumor Necrosis Factor Receptor Superfamily Member 21, is a cell membrane protein belonging to the TNF receptor superfamily. It plays a role in promoting cell apoptosis by activating the NF-kappaB pathway, which leads to the activation of caspase 3 and caspase 6, resulting in cell degeneration. TNFRSF21 binds to the N-terminal region of APP in neuronal cell bodies and axons, contributing to apoptosis. Additionally, it participates in signaling cascades triggered by T-cell receptor stimulation.
Produced in Sf9 insect cells using a baculovirus expression system, TNFRSF21 is a single, glycosylated polypeptide chain consisting of 547 amino acids (residues 42-349a.a.). It has a molecular weight of 60.4 kDa, although it may appear between 50-70 kDa on SDS-PAGE due to glycosylation. The protein is expressed with a C-terminal 239 amino acid His-tag to facilitate purification by proprietary chromatographic techniques.
The product is a sterile-filtered, colorless solution.
TNFRSF21 protein is supplied as a 1 mg/ml solution in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 0.1 mM PMSF.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein such as 0.1% HSA or BSA is recommended. Avoid repeated freeze-thaw cycles.
The purity of TNFRSF21 is greater than 85.0% as determined by SDS-PAGE analysis.
Tumor necrosis factor receptor superfamily member 21, BM-018, CD358, DR6, Death receptor 6.
Sf9, Baculovirus cells.
QPEQKASNLI GTYRHVDRAT GQVLTCDKCP AGTYVSEHCT NTSLRVCSSC PVGTFTRHEN GIEKCHDCSQ PCPWPMIEKL PCAALTDREC TCPPGMFQSN ATCAPHTVCP VGWGVRKKGT ETEDVRCKQC ARGTFSDVPS SVMKCKAYTD CLSQNLVVIK PGTKETDNVC GTLPSFSSST SPSPGTAIFP RPEHMETHEV PSSTYVPKGM NSTESNSSAS VRPKVLSSIQ EGTVPDNTSS ARGKEDVNKT LPNLQVVNHQ QGPHHRHILK LLPSMEATGG EKSSTPIKGP KRGHPRQNLH KHFDINEHLE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH.
Product Science Overview
TNFRSF21 is involved in various cellular processes, including the activation of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase 8 (MAPK8), also known as c-Jun N-terminal kinase 1 (JNK1) . It is known to induce cell apoptosis through its death domain, which interacts with the tumor necrosis factor receptor type 1-associated death domain (TRADD) protein . This interaction is essential for mediating signal transduction of tumor necrosis factor receptors.
TNFRSF21 promotes apoptosis, possibly via a pathway that involves the activation of NF-κB . It can also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm . This protein plays a significant role in neuronal apoptosis, including apoptosis in response to amyloid peptides derived from amyloid precursor protein (APP) . It is required for both normal cell body death and axonal pruning .
TNFRSF21 is involved in T-helper cell activation and may play a role in inflammation and immune regulation . Knockout studies in mice suggest that this gene is crucial for the proper functioning of the immune system . The protein encoded by this gene is also associated with various diseases, including arterial calcification, generalized of infancy, and spindle cell liposarcoma .
Recombinant human TNFRSF21 is produced using recombinant DNA technology, which involves inserting the gene encoding TNFRSF21 into a suitable expression system, such as bacteria, yeast, or mammalian cells. This allows for the large-scale production of the protein for research and therapeutic purposes.
