TNFRSF10B Human, Sf9
Sf9, Insect cells.
TNFRSF10B, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2.
Greater than 90% as determined by SDS-PAGE.
Description
TNFRSF10B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 394 amino acids (56-210a.a.) and having a molecular mass of 43.9kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
TNFRSF10B is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
TNFRSF10B, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2.
Sf9, Insect cells.
ITQQDLAPQQ RAAPQQKRSS PSEGLCPPGH HISEDGRDCI SCKYGQDYST HWNDLLFCLR CTRCDSGEVE LSPCTTTRNT VCQCEEGTFR EEDSPEMCRK CRTGCPRGMV KVGDCTPWSD IECVHKESGT KHSGEVPAVE ETVTSSPGTP ASPCSLEPKS CDKTHTCPPC PAPELLGGPS
VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ
GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH.
Product Science Overview
TNFRSF10B contains an intracellular death domain, which is crucial for its role in apoptosis (programmed cell death). The receptor is activated by binding to the tumor necrosis factor-related apoptosis-inducing ligand (TNFSF10/TRAIL/APO-2L) . Upon activation, TNFRSF10B transduces an apoptosis signal, leading to the formation of the death-inducing signaling complex (DISC). This complex recruits and activates caspase-8, initiating a cascade of caspases that mediate apoptosis .
TNFRSF10B plays a significant role in regulating apoptosis, which is essential for maintaining cellular homeostasis and preventing tumor formation. Mutations or deregulation of this gene can lead to various cancers, including squamous cell carcinoma of the head and neck . The receptor’s ability to induce apoptosis makes it a potential target for cancer therapies, as activating TNFRSF10B can selectively induce death in cancer cells while sparing normal cells .
The recombinant form of TNFRSF10B, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 394 amino acids and has a molecular mass of approximately 43.9 kDa . This recombinant protein is used in various research applications, including studying the mechanisms of apoptosis and developing potential therapeutic agents .
Recombinant TNFRSF10B is valuable in research for understanding the pathways involved in apoptosis and cancer progression. It is also used in cytotoxicity assays to measure its ability to inhibit the growth of cancer cells. The recombinant protein’s biological activity is measured by its ability to inhibit cytotoxicity in human T lymphocytes, with an effective dose (ED50) of less than or equal to 5 ng/ml when used with TRAIL .
