TNFRSF10A Human Fc
TRAIL Receptor-1 Human Recombinant Fc
Cat. No.
BT27212
Source
Escherichia Coli.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 10a, TRAILR1, APO2, DR4, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261, TNFRSF10A.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TRAIL R1 Fc Human Recombinant (24-240) produced in E.Coli is a non-glycosylated, disulfide-linked homodimer containing two polypeptide chains of 217 amino acids each and having a molecular mass of 41kDa [including tag] each chain.
TRAILR1 is fused to a 236 amino acid Fc tag at C-terminal and purified by proprietary chromatographic techniques.
TRAILR1 is fused to a 236 amino acid Fc tag at C-terminal and purified by proprietary chromatographic techniques.
Product Specs
Introduction
TNFRSF10A, a human-specific protein, is a type 1 transmembrane receptor belonging to the TNF receptor superfamily. This receptor is absent in rodents. Upon binding to its trimeric ligand, TRAIL, TNFRSF10A initiates apoptosis. Soluble forms of the receptor, produced through recombinant technology, can effectively inhibit TRAIL-mediated apoptosis. Expression of TNFRSF10A is commonly observed in cells experiencing damage, infection, or malignant transformation. This receptor plays a critical role in immune surveillance by selectively inducing apoptosis in cancerous cells while sparing healthy ones.
Description
Recombinant human TRAIL R1 Fc (amino acids 24-240) is produced in E. coli. This non-glycosylated protein forms a disulfide-linked homodimer, with each chain comprising 217 amino acids. The molecular weight of each chain, including the tag, is 41 kDa. TRAILR1 is fused to a 236 amino acid Fc tag at its C-terminus. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
The product is supplied as a sterile, white, lyophilized powder.
Formulation
The protein was lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS).
Solubility
To reconstitute the lyophilized TNFRSF10A, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized TNFRSF10A remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the desiccated product at temperatures below -18°C. After reconstitution, TRAIL-R1 should be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C is recommended. To prevent protein degradation, the addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is greater than 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The protein exhibits an ED₅₀ of less than 1.25 ng/ml when tested for its ability to inhibit TRAIL-induced cytotoxicity in mouse L-929 cells.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 10a, TRAILR1, APO2, DR4, TNF-Related Apoptosis-Inducing Ligand Receptor 1, Death Receptor 4, TRAIL Receptor 1, TRAIL-R1, Tumor Necrosis Factor Receptor Superfamily Member 10a Variant 2, Tumor Necrosis Factor Receptor Superfamily Member 10A, Cytotoxic TRAIL Receptor, CD261 Antigen, TRAILR-1, CD261, TNFRSF10A.
Source
Escherichia Coli.
Amino Acid Sequence
ASGTEAAAAT PSKVWGSSAG RIEPRGGGRG ALPTSMGQHG PSARARAGRA PGPRPAREAS PRLRVHKTFK FVVVGVLLQV VPSSAATIKL HDQSIGTQQW EHSPLGELCP PGSHRSERPG ACNRCTEGVG YTNASNNLFA CLPCTACKSD EEERSPCTTT RNTACQCKPG TFRNDNSAEM CRKCSTGCPR GMVKVKDCTP WSDIECVHKE SGNGHNIEGR MDPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK.
Product Science Overview
Structure and Function
TRAIL Receptor-1 is a type 1 transmembrane protein that consists of:
- An extracellular domain containing cysteine-rich motifs
- A transmembrane domain
- An intracellular death domain
When the trimeric ligand TRAIL (TNFSF10) binds to TRAIL Receptor-1, it triggers the formation of a death-inducing signaling complex (DISC). This complex recruits adaptor proteins such as Fas-associated death domain (FADD) and initiates the activation of caspase-8, leading to apoptosis .
Recombinant Forms
Applications in Cancer Research
TRAIL Receptor-1 has garnered significant attention in cancer research due to its ability to selectively induce apoptosis in cancer cells while sparing normal cells . This selective cytotoxicity makes it a promising target for cancer therapies. Agonistic monoclonal antibodies against TRAIL Receptor-1 have been developed to induce apoptosis in the absence of TRAIL, offering potential therapeutic strategies for various cancers .
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