TNF B Human, Sf9
Description
TNFB is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The human recombinant TNF-β produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 180 amino acids (35-205a.a.) and has a molecular mass of approximately 19.7 kDa . The recombinant protein is fused with a 6 amino acids His tag at the C-terminus and is purified using proprietary chromatographic techniques .
TNF-β mediates a wide range of biological activities, including:
- Inflammatory Responses: TNF-β is highly inducible and secreted by lymphocytes. It forms homotrimers and heterotrimers with lymphotoxin-beta, anchoring it to the cell surface .
- Immunostimulatory and Antiviral Responses: It plays a significant role in stimulating the immune system and combating viral infections .
- Apoptosis: TNF-β is involved in the process of programmed cell death, which is essential for maintaining cellular homeostasis and eliminating cancerous cells .
- Development of Secondary Lymphoid Organs: TNF-β is crucial for the formation of secondary lymphoid organs such as lymph nodes and Peyer’s patches during development .
The recombinant TNF-β protein is used extensively in laboratory research to study its effects on various cell types and its role in different biological processes. It is typically stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
The purity of the recombinant TNF-β protein is greater than 90.0% as determined by SDS-PAGE . Its biological activity is measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The effective dose (ED50) for this effect is ≤ 1 ng/ml .
