TMOD3 Human

Tropomodulin 3 Human Recombinant
Cat. No.
BT25579
Source
Escherichia Coli.
Synonyms
Tropomodulin-3, Ubiquitous tropomodulin, U-Tmod, TMOD3, UTMOD.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TMOD3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 376 amino acids (1-352 a.a) and having a molecular mass of 42kDa.
TMOD3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Tropomodulin 3 (TMOD3) belongs to the tropomodulin family and plays a crucial role in regulating the actin cytoskeleton. Its primary function is to prevent the elongation and depolymerization of actin filaments at their pointed ends. This capping activity is vital for various dynamic cellular processes. While TMOD3 is known to negatively regulate cell migration, the precise mechanisms underlying its cellular functions remain to be fully elucidated. The Tmod/TM complex is involved in the formation of short actin protofilaments, which subsequently define the membrane skeleton's geometry.
Description
Recombinant human TMOD3, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 376 amino acids (with the first 352 amino acids being part of the TMOD3 sequence) and has a molecular weight of 42 kDa. For purification and detection purposes, a 24 amino acid His-tag is fused to the N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The provided TMOD3 protein solution has a concentration of 0.25 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 0.15M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the protein solution should be kept at 4°C. Long-term storage requires freezing at -20°C. To enhance stability during extended storage, adding a carrier protein (either 0.1% HSA or BSA) is recommended. Repeated freezing and thawing should be avoided.
Purity
The purity of TMOD3 is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Tropomodulin-3, Ubiquitous tropomodulin, U-Tmod, TMOD3, UTMOD.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMALPFR KDLEKYKDLD EDELLGNLSE TELKQLETVL DDLDPENALL PAGFRQKNQT SKSTTGPFDR EHLLSYLEKE ALEHKDREDY VPYTGEKKGK IFIPKQKPVQ TFTEEKVSLD PELEEALTSA SDTELCDLAA ILGMHNLITN TKFCNIMGSS NGVDQEHFSN VVKGEKILPV FDEPPNPTNV EESLKRTKEN DAHLVEVNLN NIKNIPIPTL KDFAKALETN THVKCFSLAA TRSNDPVATA FAEMLKVNKT LKSLNVESNF ITGVGILALI DALRDNETLA ELKIDNQRQQ LGTAVELEMA KMLEENTNIL KFGYQFTQQG PRTRAANAIT KNNDLVRKRR VEGDHQ.

Product Science Overview

Introduction

Tropomodulin 3 (TMOD3) is a member of the tropomodulin family, which plays a crucial role in the regulation of the actin cytoskeleton. The actin cytoskeleton is essential for various dynamic cellular functions, including cell shape, motility, and division . TMOD3 is also known by other names such as Ubiquitous tropomodulin (U-Tmod) and TMOD3 .

Structure and Expression

Recombinant Human Tropomodulin 3 is a full-length protein consisting of 352 amino acids. It is typically expressed in Escherichia coli and purified to a high degree of purity (>80%) suitable for applications such as SDS-PAGE and mass spectrometry (MS) . The protein often includes an N-terminal His-tag to facilitate purification and detection .

Function

TMOD3 functions as a capping protein that binds to the pointed ends of actin filaments, thereby regulating their elongation and depolymerization . This capping activity is critical for maintaining the stability and organization of the actin cytoskeleton, which in turn influences various cellular processes .

Biological Significance

The ability of TMOD3 to bind both actin filaments and monomers is central to its role in cellular functions. By capping the pointed ends of actin filaments, TMOD3 helps define the geometry of the membrane skeleton, contributing to the structural integrity and function of cells .

Applications

Recombinant Human Tropomodulin 3 is widely used in research to study the dynamics of the actin cytoskeleton and its role in cellular processes. It is also utilized in various biochemical assays, including SDS-PAGE and MS, to analyze protein interactions and functions .

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