TMEM27 Human
Description
Product Specs
TMEM27, also known as Transmembrane Protein 27, is a type 1 transmembrane protein. This protein is essential for the transportation of amino acid transporters to the apical brush border of proximal tubules. TMEM27 binds to these amino acid transporters and plays a crucial role in controlling their presence on the plasma membrane. In beta cells, the extracellular domain of TMEM27 undergoes cleavage and is released from the cell surface.
To prepare a working stock solution, it is recommended to reconstitute the lyophilized protein by adding 0.1M Acetate buffer at pH 4.0. This should result in a solution with an approximate concentration of 0.5mg/ml. Allow the lyophilized pellet to dissolve fully at a temperature of 37°C. If a higher pH value is required, it is advised to dilute the solution extensively with the appropriate buffer to achieve a concentration of 10µg/ml. Please note that the solubility of this antigen is limited at higher concentrations. It is important to note that TMEM27 is not sterile. Before using this product in cell culture, it is essential to filter it through an appropriate sterile filter.
Product Science Overview
Transmembrane Protein 27 (TMEM27), also known as collectrin, is a membrane protein that plays a significant role in various physiological processes. It is particularly notable for its involvement in pancreatic beta-cell function and its potential as a biomarker for beta-cell mass.
TMEM27 is a type I transmembrane protein, meaning it spans the cell membrane with a single transmembrane domain. The protein is primarily expressed in the kidneys and pancreatic islets. In pancreatic beta cells, TMEM27 is involved in insulin exocytosis and cell proliferation. It is cleaved and shed by these cells, which has led to its investigation as a potential biomarker for beta-cell mass .
TMEM27 has been studied extensively for its role in pancreatic beta cells, which are responsible for insulin production. Research has shown that TMEM27 mRNA levels are lower in diabetic donors compared to healthy controls. This suggests that TMEM27 expression is correlated with insulin secretion and beta-cell function . Overproduction of TMEM27 in beta cells has been found to enhance glucose-induced insulin secretion, although its effects on cell proliferation are modest .
The potential of TMEM27 as a biomarker for beta-cell mass has been a topic of interest. However, its utility is challenged by the fact that TMEM27 is also cleaved and shed by renal proximal tubular cells, not just pancreatic beta cells . This non-specific cleavage raises questions about its specificity and reliability as a biomarker for beta-cell mass.
Human recombinant TMEM27 is produced using mammalian cell expression systems, such as HEK-293 cells. These systems are widely used for the production of recombinant proteins due to their ability to perform complex post-translational modifications . The recombinant form of TMEM27 is used in various research applications to study its structure, function, and potential therapeutic uses.
