TINAGL1 Human, Sf9
Sf9, Baculovirus cells.
Tubulointerstitial nephritis antigen-like isoform 1, TINAGL1, Tubulointerstitial nephritis antigen-like, Glucocorticoid-inducible protein 5, Oxidized LDL-responsive gene 2 protein, OLRG-2, Tubulointerstitial nephritis antigen-related protein, TIN Ag-related protein, TIN-Ag-RP, GIS5, LCN7, OLRG2, TINAGL, PP6614, PSEC0088, UNQ204/PRO230.
Greater than 90.0% as determined by SDS-PAGE.
Description
TINAGL1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 455 amino acids (22-467a.a.) and having a molecular mass of 51.2kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). TINAGL1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Tubulointerstitial Nephritis Antigen Like 1, also referred to as TINAGL1, exhibits sequence similarity to tubulointerstitial nephritis antigen. This secreted glycoprotein is targeted by antibodies in various immune-mediated tubulointerstitial nephritis cases. Currently, three transcript variants encoding distinct isoforms of TINAGL1 have been identified.
Recombinant human TINAGL1, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein comprises 455 amino acids (22-467a.a.), resulting in a molecular mass of 51.2 kDa. Notably, the observed molecular size on SDS-PAGE may range from 50 to 70 kDa. The TINAGL1 protein is expressed with a C-terminal 6-amino acid Histidine tag and purified using proprietary chromatographic methods.
The TINAGL1 protein solution is supplied at a concentration of 0.25 mg/ml in a buffer consisting of Phosphate Buffered Saline (pH 7.4), 30% glycerol, and 1mM DTT.
The purity of the TINAGL1 protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Tubulointerstitial nephritis antigen-like isoform 1, TINAGL1, Tubulointerstitial nephritis antigen-like, Glucocorticoid-inducible protein 5, Oxidized LDL-responsive gene 2 protein, OLRG-2, Tubulointerstitial nephritis antigen-related protein, TIN Ag-related protein, TIN-Ag-RP, GIS5, LCN7, OLRG2, TINAGL, PP6614, PSEC0088, UNQ204/PRO230.
Sf9, Baculovirus cells.
ADLAQQGRGR RELAPGLHLR GIRDAGGRYC QEQDLCCRGR ADDCALPYLG AICYCDLFCN RTVSDCCPDF WDFCLGVPPP FPPIQGCMHG GRIYPVLGTY WDNCNRCTCQ ENRQWQCDQE PCLVDPDMIK AINQGNYGWQ AGNHSAFWGM TLDEGIRYRL GTIRPSSSVM NMHEIYTVLN PGEVLPTAFE ASEKWPNLIH EPLDQGNCAG SWAFSTAAVA SDRVSIHSLG HMTPVLSPQN LLSCDTHQQQ GCRGGRLDGA WWFLRRRGVV SDHCYPFSGR ERDEAGPAPP CMMHSRAMGR GKRQATAHCP NSYVNNNDIY QVTPVYRLGS NDKEIMKELM ENGPVQALME VHEDFFLYKG GIYSHTPVSL GRPERYRRHG THSVKITGWG EETLPDGRTL KYWTAANSWG PAWGERGHFR IVRGVNECDI ESFVLGVWGR VGMEDMGHHH HHHHH.
Product Science Overview
TINAGL1 is implicated in several biological functions and processes. It is believed to play a role in adrenocortical zonation and mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells . Additionally, TINAGL1 has been proposed to protect against cancer and contribute to pathological abnormalities in tumors .
TINAGL1 has been associated with various diseases, including colorectal adenocarcinoma . Research has shown that TINAGL1 secreted by cancer-associated fibroblasts (CAFs) contributes to the progression of diffuse-type gastric cancers through interaction with integrin β1 . This interaction promotes tumor progression by enhancing the phosphorylation of focal adhesion kinase (FAK) and mesenchymal marker expression in cancer cells .
The recombinant form of TINAGL1, produced in Sf9 insect cells, is used for various research purposes. This recombinant protein is valuable for studying the biological functions and clinical significance of TINAGL1 in different disease contexts.
